Anti-HAX1 antibody (ab78939)
Key features and details
- Rabbit polyclonal to HAX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HAX1 antibody
See all HAX1 primary antibodies -
Description
Rabbit polyclonal to HAX1 -
Host species
Rabbit -
Specificity
At least four isoforms of HAX1 are known to exist. ab78939 is expected to recognize the longest isoform (HAX1a) as well as the shortest. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human HAX1. A 15 amino acid synthetic peptide near the amino terminus of human HAX1. The immunogen is located within the first 50 amino acids of Hax1a.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab78939 was purified by affinity chromatography via a peptide column -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 32 kDa.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 32 kDa. |
Target
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Function
Promotes cell survival. Potentiates GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. May regulate intracellular calcium pools. -
Tissue specificity
Ubiquitous. Up-regulated in oral cancers. -
Involvement in disease
Defects in HAX1 are the cause of neutropenia severe congenital autosomal recessive type 3 (SCN3) [MIM:610738]; also known as Kostmann disease. A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Some patients affected by severe congenital neutropenia type 3 have neurological manifestations such as psychomotor retardation and seizures. Note=The clinical phenotype due to HAX1 deficiency appears to depend on the localization of the mutations and their influence on the transcript variants. Mutations affecting exclusively isoform 1 are associated with isolated congenital neutropenia, whereas mutations affecting both isoform 1 and isoform 5 are associated with additional neurologic symptoms. -
Sequence similarities
Belongs to the HAX1 family. -
Post-translational
modificationsProteolytically cleaved by caspase-3 during apoptosis. -
Cellular localization
Mitochondrion. Endoplasmic reticulum. Nucleus membrane. Cytoplasmic vesicle. Sarcoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 10456 Human
- Omim: 605998 Human
- SwissProt: O00165 Human
- Unigene: 199625 Human
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Alternative names
- FLJ17042 antibody
- FLJ18492 antibody
- FLJ93803 antibody
see all
Images
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Lane 1 : Anti-HAX1 antibody (ab78939) at 1 µg/ml
Lane 2 : Anti-HAX1 antibody (ab78939) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 32 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab78939 has been referenced in 3 publications.
- Chen Y et al. Prevotellaceae produces butyrate to alleviate PD-1/PD-L1 inhibitor-related cardiotoxicity via PPARα-CYP4X1 axis in colonic macrophages. J Exp Clin Cancer Res 41:1 (2022). PubMed: 34980222
- Zha Z & Li J MicroRNA-125a-5p regulates liver cancer cell growth, migration and invasion and EMT by targeting HAX1. Int J Mol Med 46:1849-1861 (2020). PubMed: 33000203
- Rivers-Auty J et al. Redefining the ancestral origins of the interleukin-1 superfamily. Nat Commun 9:1156 (2018). PubMed: 29559685