Recombinant
RabMAb

Recombinant Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (ab79541)

Overview

  • Product name

    Anti-HB9/HLXB9/MNX1 antibody [EPR3342]
    See all HB9/HLXB9/MNX1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3342] to HB9/HLXB9/MNX1
  • Host species

    Rabbit
  • Specificity

    There have been conflicting results with this antibody in IHC-P. PMID 21484430 reported specific labeling in IHC-P, but internally we were unable to reproduce these results. Thus, we have removed IHC-P as a guaranteed application, and we welcome any further feedback from customers using this antibody in IHC-P.
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human HB9/HLXB9/MNX1 aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Molt-4 and Raji cell lysates.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

     This product was previously labelled as HB9/HLXB9

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab79541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
IP 1/50.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Putative transcription factor involved in pancreas development and function.
    • Tissue specificity

      Expressed in lymphoid and pancreatic tissues.
    • Involvement in disease

      Defects in MNX1 are a cause of Currarino syndrome (CURRAS) [MIM:176450]. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait.
    • Sequence similarities

      Contains 1 homeobox DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • HB 9 antibody
      • HB9 antibody
      • HLXB 9 antibody
      • HLXB9 antibody
      • Homeo box HB9 antibody
      • Homeobox HB9 antibody
      • Homeobox protein HB9 antibody
      • HOXHB9 antibody
      • MNX1 antibody
      • MNX1_HUMAN antibody
      • Motor neuron and pancreas homeobox 1 antibody
      • Motor neuron and pancreas homeobox protein 1 antibody
      • Sacral agenesis autosomal dominant (Currarino triad) antibody
      • SCRA 1 antibody
      • SCRA1 antibody
      see all

    Images

    • All lanes : Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (ab79541) at 1/200000 dilution

      Lane 1 : Molt-4 cell lysate
      Lane 2 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 41 kDa
      Observed band size: 48 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Egawa N  et al. Drug screening for ALS using patient-specific induced pluripotent stem cells. Sci Transl Med 4:145ra104 (2012). Human . Read more (PubMed: 22855461) »
    • Wilkens L  et al. The homeobox gene HLXB9 is upregulated in a morphological subset of poorly differentiated hepatocellular carcinoma. Virchows Arch 458:697-708 (2011). IHC-P, WB ; Human . Read more (PubMed: 21484430) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    I will send the IHC protocol for ab59795 when I receive it, hoepfully in the next day or two. As I mentioned, Abcam does not validate this antibody for IHC in-house. We do not have free samples of any of our antibodies, but ab59795 is covered by our Abpromise guarantee for this application (IHC on paraffin-embedded tissue sections) and this species (human). If I cannot help you obtain a satisfactory result, I will be happy to look into replacing this. However, I am concerned about the possility that this transcription factor is not present in prostate. Do you have any other tissues you can include as apositive control, in particular pancreas?

    Read More

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