Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3342] to HB9/HLXB9/MNX1
- Suitable for: WB
- Reacts with: Mouse, Human
Product nameAnti-HB9/HLXB9/MNX1 antibody [EPR3342]
See all HB9/HLXB9/MNX1 primary antibodies
DescriptionRabbit monoclonal [EPR3342] to HB9/HLXB9/MNX1
SpecificityThere have been conflicting results with this antibody in IHC-P. PMID 21484430 reported specific labeling in IHC-P, but internally we were unable to reproduce these results. Thus, we have removed IHC-P as a guaranteed application, and we welcome any further feedback from customers using this antibody in IHC-P.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human HB9/HLXB9/MNX1 aa 250-350 (C terminal). The exact sequence is proprietary.
- Molt-4 and Raji cell lysates.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as HB9/HLXB9
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab79541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).|
FunctionPutative transcription factor involved in pancreas development and function.
Tissue specificityExpressed in lymphoid and pancreatic tissues.
Involvement in diseaseDefects in MNX1 are a cause of Currarino syndrome (CURRAS) [MIM:176450]. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait.
Sequence similaritiesContains 1 homeobox DNA-binding domain.
- Information by UniProt
- HB 9 antibody
- HB9 antibody
- HLXB 9 antibody
All lanes : Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (ab79541) at 1/200000 dilution
Lane 1 : Molt-4 cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
ab79541 has been referenced in 5 publications.
- Park S et al. Differentiation of Motor Neuron-Like Cells from Tonsil-Derived Mesenchymal Stem Cells and Their Possible Application to Neuromuscular Junction Formation. Int J Mol Sci 20:N/A (2019). PubMed: 31159418
- Egawa N et al. Drug screening for ALS using patient-specific induced pluripotent stem cells. Sci Transl Med 4:145ra104 (2012). Human . PubMed: 22855461
- Ruggiu M et al. A role for SMN exon 7 splicing in the selective vulnerability of motor neurons in spinal muscular atrophy. Mol Cell Biol 32:126-38 (2012). PubMed: 22037760
- Wilkens L et al. The homeobox gene HLXB9 is upregulated in a morphological subset of poorly differentiated hepatocellular carcinoma. Virchows Arch 458:697-708 (2011). IHC-P, WB ; Human . PubMed: 21484430
- Wu CY et al. Proteomic assessment of a cell model of spinal muscular atrophy. BMC Neurosci 12:25 (2011). PubMed: 21385431