• Product name

    Anti-HBEGF/DTR antibody [4G10]
    See all HBEGF/DTR primary antibodies
  • Description

    Mouse monoclonal [4G10] to HBEGF/DTR
  • Host species

  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human HBEGF/DTR.

  • Epitope

    EGF domain
  • Positive control

    • Vero cell extract carrrying human HBEGF/DTR expression vector.
  • General notes

     This product was previously labelled as HB EGF




Our Abpromise guarantee covers the use of ab66792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Use at 2μg/ml

WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23, 40 kDa).
ICC/IF Use a concentration of 5 - 10 µg/ml.


  • Function

    May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
  • Sequence similarities

    Contains 1 EGF-like domain.
  • Post-translational

    Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
    O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
  • Cellular localization

    Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
  • Information by UniProt
  • Database links

  • Alternative names

    • diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
    • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
    • Diphtheria toxin receptor antibody
    • DT R antibody
    • DT-R antibody
    • DTR antibody
    • DTS antibody
    • DTSF antibody
    • HB-EGF antibody
    • HBEGF antibody
    • HBEGF_HUMAN antibody
    • HEGFL antibody
    • Heparin binding EGF like growth factor antibody
    • Heparin binding epidermal growth factor antibody
    • Heparin binding epidermal growth factor like growth factor antibody
    • Heparin-binding EGF-like growth factor antibody
    • Proheparin binding EGF like growth factor antibody
    see all


  • All lanes : Anti-HBEGF/DTR antibody [4G10] (ab66792) at 1 µg/ml

    Lane 1 : Vero cell extract
    Lane 2 : Vero cell extract from cells
    carrrying human HBEGF/DTR expression vector.
    Lane 3 : Vero cell extract from cells carrying
    mouse HBEGF/DTR expression vector

    Predicted band size: 23, 40 kDa
    Observed band size: 20-28 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.

  • Lane 1: Vero cell extract, 1μg
    Lane 2: Vero cells carrying Human HBEGF/DTR expression vector, 1μg
    Lane 3: Vero Cells carrying mouse HBEGF/DTR expression vector, 1μg

    Ab66792 immunoprecipitating HBEGF/DTR in Vero Cells. For western blotting, a HRP conjugated streptavidin was used as the secondary antibody; cell surface was biotinylated.

  • These images show Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR expression vector (right hand image). Cells treated with ab 66792, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.


This product has been referenced in:

  • Shen L  et al. C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers. Exp Ther Med 13:3021-3031 (2017). Read more (PubMed: 28587375) »
  • Tang XH  et al. The anti-tumor effect of cross-reacting material 197, an inhibitor of heparin-binding EGF-like growth factor, in human resistant ovarian cancer. Biochem Biophys Res Commun 422:676-80 (2012). Human . Read more (PubMed: 22609777) »
See all 2 Publications for this product

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