Overview

  • Product name

    Anti-HBEGF/DTR antibody [4G10]
    See all HBEGF/DTR primary antibodies
  • Description

    Mouse monoclonal [4G10] to HBEGF/DTR
  • Host species

    Mouse
  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human HBEGF/DTR.

  • Epitope

    EGF domain
  • Positive control

    • Vero cell extract carrrying human HBEGF/DTR expression vector.
  • General notes

     This product was previously labelled as HB EGF

     

Properties

Applications

Our Abpromise guarantee covers the use of ab66792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Use at 2μg/ml

WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23, 40 kDa).
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • Function

    May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
  • Sequence similarities

    Contains 1 EGF-like domain.
  • Post-translational
    modifications

    Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
    O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
  • Cellular localization

    Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
  • Information by UniProt
  • Database links

  • Alternative names

    • diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
    • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
    • Diphtheria toxin receptor antibody
    • DT R antibody
    • DT-R antibody
    • DTR antibody
    • DTS antibody
    • DTSF antibody
    • HB-EGF antibody
    • HBEGF antibody
    • HBEGF_HUMAN antibody
    • HEGFL antibody
    • Heparin binding EGF like growth factor antibody
    • Heparin binding epidermal growth factor antibody
    • Heparin binding epidermal growth factor like growth factor antibody
    • Heparin-binding EGF-like growth factor antibody
    • Proheparin binding EGF like growth factor antibody
    see all

Images

  • All lanes : Anti-HBEGF/DTR antibody [4G10] (ab66792) at 1 µg/ml

    Lane 1 : Vero cell extract
    Lane 2 : Vero cell extract from cells
    carrrying human HBEGF/DTR expression vector.
    Lane 3 : Vero cell extract from cells carrying
    mouse HBEGF/DTR expression vector

    Predicted band size: 23, 40 kDa
    Observed band size: 20-28 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.

  • Lane 1: Vero cell extract, 1μg
    Lane 2: Vero cells carrying Human HBEGF/DTR expression vector, 1μg
    Lane 3: Vero Cells carrying mouse HBEGF/DTR expression vector, 1μg

    Ab66792 immunoprecipitating HBEGF/DTR in Vero Cells. For western blotting, a HRP conjugated streptavidin was used as the secondary antibody; cell surface was biotinylated.

  • These images show Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR expression vector (right hand image). Cells treated with ab 66792, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.

References

This product has been referenced in:

  • Shen L  et al. C-X-C motif chemokine ligand 8 promotes endothelial cell homing via the Akt-signal transducer and activator of transcription pathway to accelerate healing of ischemic and hypoxic skin ulcers. Exp Ther Med 13:3021-3031 (2017). Read more (PubMed: 28587375) »
  • Tang XH  et al. The anti-tumor effect of cross-reacting material 197, an inhibitor of heparin-binding EGF-like growth factor, in human resistant ovarian cancer. Biochem Biophys Res Commun 422:676-80 (2012). Human . Read more (PubMed: 22609777) »
See all 2 Publications for this product

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