Overview

  • Product name

    Anti-HBEGF/DTR antibody [4G10] (Biotin)
    See all HBEGF/DTR primary antibodies
  • Description

    Mouse monoclonal [4G10] to HBEGF/DTR (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: IP, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human HBEGF/DTR.

  • Epitope

    EGF domain
  • Positive control

    • Vero cell extract carrying human HG-EGF expression vector.
  • General notes

    Biotin/IgG = 7.5

     This product was previously labelled as HB EGF

     

Properties

Applications

Our Abpromise guarantee covers the use of ab66793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
  • Sequence similarities

    Contains 1 EGF-like domain.
  • Post-translational
    modifications

    Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
    O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
  • Cellular localization

    Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
  • Information by UniProt
  • Database links

  • Alternative names

    • diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
    • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
    • Diphtheria toxin receptor antibody
    • DT R antibody
    • DT-R antibody
    • DTR antibody
    • DTS antibody
    • DTSF antibody
    • HB-EGF antibody
    • HBEGF antibody
    • HBEGF_HUMAN antibody
    • HEGFL antibody
    • Heparin binding EGF like growth factor antibody
    • Heparin binding epidermal growth factor antibody
    • Heparin binding epidermal growth factor like growth factor antibody
    • Heparin-binding EGF-like growth factor antibody
    • Proheparin binding EGF like growth factor antibody
    see all

Images

  • All lanes : Anti-HBEGF/DTR antibody [4G10] (Biotin) (ab66793) at 1 µg/ml

    Lane 1 : Vero cell extract
    Lane 2 : Vero cell extract from cells
    carrrying human HBEGF/DTR expression vector.
    Lane 3 : Vero cell extract from cells carrying
    mouse HBEGF/DTR expression vector.

    Predicted band size: 23 kDa
    Observed band size: 20-28 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.

  • These images shows Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR  expression vector (right hand image). Cells treated with ab66793, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.

  • All lanes : HBEGF/DTR  antibody [4G10](biotin) - Azide free (ab66793) at 1 µg/ml. Lane 1 : Vero cell extract Lane 2 : Vero cell extract from cells carrrying human HBEGF/DTR  expression vector. Lane 3 : Vero cell extract from cells carrying mouse HBEGF/DTR expression vector Predicted band size : 23 kDa Observed band size : 20-38 kDa Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands. This image shows Immunoprecipitation of HBEGF/DTR followed by Western blotting/SDS PAGE; the cell surface was biotinylated.

References

ab66793 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for your inquiry. You probably could use an anti-mouse secondary antibody with ab66793 since we know it worked well with a Cy3-conjugated secondary antibody. But, there are 7.5 biotin molecules per IgG1 molecule, and that could cause a lot of hindrance to block the binding of the secondary antibody. We do have anti-biotin conjugated antibodies (if that's what you are looking for), such as Anti-Biotin (Alkaline Phosphatase) ab6652, Anti-Biotin (HRP) ab19221, and Anti-Biotin (FITC) ab6650. Click here (or use the following: https://www.abcam.com/index.html?datasheet=6652). Click here (or use the following: https://www.abcam.com/index.html?datasheet=19221). Click here (or use the following: https://www.abcam.com/index.html?datasheet=6650). What is probably best to do is to use a strepatvidin conjugate to bind the biotin on ab66793. We have two (peroxidase and AP). https://www.abcam.com/Streptavidin-Peroxidase-Ready-to-Use-ab64269.html https://www.abcam.com/Streptavidin-Alkaline-Phosphatase-Ready-to-Use-ab64268.html I hope this information helps. Please contact us with any other questions.

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Answer

We could not find the sequence of Macaque monkey HB-EGF in databases. However, since the green monkey HB-EGF sequence has 97% identity to that of human, there is a good chance that ab66793 react with Macaque HB-EGF. Though the human HB-EGF has 80% identity with that of mouse and ab66793 did not react with mouse HB-EGF.

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