Key features and details
- Mouse monoclonal [4G10] to HBEGF/DTR (Biotin)
- Suitable for: IP, ICC/IF, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Product nameAnti-HBEGF/DTR antibody [4G10] (Biotin)
See all HBEGF/DTR primary antibodies
DescriptionMouse monoclonal [4G10] to HBEGF/DTR (Biotin)
Tested applicationsSuitable for: IP, ICC/IF, WBmore details
Species reactivityReacts with: Human
Does not react with: Mouse
Recombinant fragment corresponding to Human HBEGF/DTR.
- Vero cell extract carrying human HG-EGF expression vector.
Biotin/IgG = 7.5
This product was previously labelled as HB EGF
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesProduced in serum-free medium and purified by combination of chromatography.
Our Abpromise guarantee covers the use of ab66793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
|WB||Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa).|
FunctionMay be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
Sequence similaritiesContains 1 EGF-like domain.
modificationsSeveral N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
Cellular localizationCell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
- Information by UniProt
- diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
- diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
- Diphtheria toxin receptor antibody
All lanes : Anti-HBEGF/DTR antibody [4G10] (Biotin) (ab66793) at 1 µg/ml
Lane 1 : Vero cell extract
Lane 2 : Vero cell extract from cells
carrrying human HBEGF/DTR expression vector.
Lane 3 : Vero cell extract from cells carrying
mouse HBEGF/DTR expression vector.
Predicted band size: 23 kDa
Observed band size: 20-28 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.
These images shows Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HBEGF/DTR expression vector (right hand image). Cells treated with ab66793, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
All lanes : HBEGF/DTR antibody [4G10](biotin) - Azide free (ab66793) at 1 µg/ml. Lane 1 : Vero cell extract Lane 2 : Vero cell extract from cells carrrying human HBEGF/DTR expression vector. Lane 3 : Vero cell extract from cells carrying mouse HBEGF/DTR expression vector Predicted band size : 23 kDa Observed band size : 20-38 kDa Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands. This image shows Immunoprecipitation of HBEGF/DTR followed by Western blotting/SDS PAGE; the cell surface was biotinylated.
ab66793 has not yet been referenced specifically in any publications.