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    hbegfdtr-antibody-ab92620.pdf

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Signal Transduction Growth Factors/Hormones EGF
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Anti-HBEGF/DTR antibody (ab92620)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (4)

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Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody (ab92620)
  • Western blot - Anti-HBEGF/DTR antibody (ab92620)

Key features and details

  • Rabbit polyclonal to HBEGF/DTR
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HBEGF/DTR antibody
    See all HBEGF/DTR primary antibodies
  • Description

    Rabbit polyclonal to HBEGF/DTR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HBEGF/DTR aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q99075
    (Peptide available as ab104379)

  • General notes

     This product was previously labelled as HB EGF

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cancer
    • Growth factors
    • EGF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab92620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
  • Sequence similarities

    Contains 1 EGF-like domain.
  • Post-translational
    modifications

    Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
    O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
  • Cellular localization

    Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
  • Target information above from: UniProt accession Q99075 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1839 Human
    • Omim: 126150 Human
    • SwissProt: Q99075 Human
    • Unigene: 799 Human
    • Alternative names

      • diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
      • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
      • Diphtheria toxin receptor antibody
      • DT R antibody
      • DT-R antibody
      • DTR antibody
      • DTS antibody
      • DTSF antibody
      • HB-EGF antibody
      • HBEGF antibody
      • HBEGF_HUMAN antibody
      • HEGFL antibody
      • Heparin binding EGF like growth factor antibody
      • Heparin binding epidermal growth factor antibody
      • Heparin binding epidermal growth factor like growth factor antibody
      • Heparin-binding EGF-like growth factor antibody
      • Proheparin binding EGF like growth factor antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody (ab92620)
      Immunocytochemistry/ Immunofluorescence - Anti-HBEGF/DTR antibody (ab92620)

      ICC/IF image of ab92620 stained MG63 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92620 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Western blot - Anti-HBEGF/DTR antibody (ab92620)
      Western blot - Anti-HBEGF/DTR antibody (ab92620)
      Anti-HBEGF/DTR antibody (ab92620) at 1 µg/ml + Human stomach tumor tissue lysate (adenocarcinoma) - total protein (ab29678) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 23 kDa


      Exposure time: 4 minutes


      The band observed at 20 kDa could potentially be a cleaved form of HB EGF due to the presence of a 19 amino acid signal peptide.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab92620? Please let us know so that we can cite the reference in this datasheet.

    ab92620 has been referenced in 4 publications.

    • Tornabene E  et al. Effects of oxygen-glucose deprivation (OGD) on barrier properties and mRNA transcript levels of selected marker proteins in brain endothelial cells/astrocyte co-cultures. PLoS One 14:e0221103 (2019). PubMed: 31425564
    • Zhang Y  et al. Hypothalamic stem cells control ageing speed partly through exosomal miRNAs. Nature 548:52-57 (2017). PubMed: 28746310
    • Gaviglio AL  et al. Heparin-binding epidermal growth factor-like growth factor promotes neuroblastoma differentiation. FASEB J 31:1903-1915 (2017). PubMed: 28174207
    • Altonsy MO  et al. Context-dependent cooperation between nuclear factor ?B (NF-?B) and the glucocorticoid receptor at a TNFAIP3 intronic enhancer: a mechanism to maintain negative feedback control of inflammation. J Biol Chem 289:8231-9 (2014). WB ; Human . PubMed: 24500711

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-HBEGF/DTR antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Primary Keratinocytes)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    40 µg
    Treatment
    microRNAs
    Specification
    Human Primary Keratinocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 03 2016

    ELISA abreview for Anti-HBEGF/DTR antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Purified protein (ab)
    Specification
    ab
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Type
    Sandwich (Capture)
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 14 2014

    IHC - Wholemount abreview for Anti-HBEGF/DTR antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Olfactory Bulb)
    Specification
    Olfactory Bulb
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 17 2013

    Western blot abreview for Anti-HBEGF/DTR antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (whole bladder)
    Gel Running Conditions
    Reduced Denaturing (Tris-HCl 10%)
    Loading amount
    60 µg
    Specification
    whole bladder
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 04 2011

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