Product nameAnti-HBEGF/DTR antibody [EPR13276(2)]
See all HBEGF/DTR primary antibodies
DescriptionRabbit monoclonal [EPR13276(2)] to HBEGF/DTR
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human HBEGF/DTR aa 50-150. The exact sequence is proprietary.
Database link: Q99075
- HeLa and K563 cell lysates.
This product was previously labelled as HB EGF
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
FunctionMay be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
Sequence similaritiesContains 1 EGF-like domain.
modificationsSeveral N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
Cellular localizationCell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
- Information by UniProt
- diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
- diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
- Diphtheria toxin receptor antibody
Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling HB EGF using ab185555 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
All lanes : Anti-HBEGF/DTR antibody [EPR13276(2)] (ab185555) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 23 kDa
This product has been referenced in:
- De Pasquale V et al. EGFR activation triggers cellular hypertrophy and lysosomal disease in NAGLU-depleted cardiomyoblasts, mimicking the hallmarks of mucopolysaccharidosis IIIB. Cell Death Dis 9:40 (2018). Read more (PubMed: 29348482) »