RecombinantRabMAb

Recombinant Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free (ab240293)

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Immunoprecipitation - Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free (ab240293)
  • Western blot - Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free (ab240293)
  • Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free (ab240293)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13276(2)] to HBEGF/DTR - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free
    See all HBEGF/DTR primary antibodies

  • Description

    Rabbit monoclonal [EPR13276(2)] to HBEGF/DTR - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab240293 is the carrier-free version of ab185555 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240293 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as HB EGF

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab240293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IP
Use at an assay dependent concentration.

Target

  • Function

    May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

  • Sequence similarities

    Contains 1 EGF-like domain.

  • Post-translational
    modifications

    Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
    O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).

  • Cellular localization

    Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
  • Information by UniProt

  • Database links

    • Alternative names

      • diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
      • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
      • Diphtheria toxin receptor antibody
      • DT R antibody
      • DT-R antibody
      • DTR antibody
      • DTS antibody
      • DTSF antibody
      • HB-EGF antibody
      • HBEGF antibody
      • HBEGF_HUMAN antibody
      • HEGFL antibody
      • Heparin binding EGF like growth factor antibody
      • Heparin binding epidermal growth factor antibody
      • Heparin binding epidermal growth factor like growth factor antibody
      • Heparin-binding EGF-like growth factor antibody
      • Proheparin binding EGF like growth factor antibody
      see all

    Images

    • Immunoprecipitation - Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free (ab240293)
      Immunoprecipitation - Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free (ab240293)

      This data was developed using ab185555, the same antibody clone in a different buffer formulation.

      Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling HB EGF using ab185555 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

    • Western blot - Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free (ab240293)
      Western blot - Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free (ab240293)
      All lanes : Anti-HBEGF/DTR antibody [EPR13276(2)] (ab185555) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 23 kDa
      Observed band size: 23 kDa



      This data was developed using ab185555, the same antibody clone in a different buffer formulation.

       

    • Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free (ab240293)
      Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free (ab240293)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240293? Please let us know so that we can cite the reference in this datasheet.

    ab240293 has not yet been referenced specifically in any publications.

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