Recombinant Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free (ab240293)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13276(2)] to HBEGF/DTR - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Rat, Human
Overview
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Product name
Anti-HBEGF/DTR antibody [EPR13276(2)] - BSA and Azide free
See all HBEGF/DTR primary antibodies -
Description
Rabbit monoclonal [EPR13276(2)] to HBEGF/DTR - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species IP HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab240293 is the carrier-free version of ab185555 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab240293 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as HB EGF
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13276(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IP |
Human
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WB |
Human
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Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. -
Sequence similarities
Contains 1 EGF-like domain. -
Post-translational
modificationsSeveral N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937). -
Cellular localization
Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor. - Information by UniProt
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Database links
- Entrez Gene: 1839 Human
- Entrez Gene: 25433 Rat
- Omim: 126150 Human
- SwissProt: Q99075 Human
- SwissProt: Q06175 Rat
- Unigene: 799 Human
- Unigene: 10148 Rat
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Alternative names
- diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
- diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
- Diphtheria toxin receptor antibody
see all
Images
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This data was developed using ab185555, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling HB EGF using ab185555 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
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All lanes : Anti-HBEGF/DTR antibody [EPR13276(2)] (ab185555) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 23 kDaThis data was developed using ab185555, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab240293 has not yet been referenced specifically in any publications.