Key features and details
- Rabbit polyclonal to HBP1 (phospho S402)
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HBP1 (phospho S402) antibody
See all HBP1 primary antibodies
DescriptionRabbit polyclonal to HBP1 (phospho S402)
Specificityab73339 detects endogenous levels of HBP1 only when phosphorylated at serine 402 (Human) or serine 404 (Mouse).
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phosphopeptide derived from human HBP1 around the phosphorylation site of serine 402 (CGSPPG)
- Extracts from A549 cells treated with PMA (125ng/ml, 30mins).
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab73339 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab73339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).|
FunctionTranscriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.
Sequence similaritiesContains 1 AXH domain.
Contains 1 HMG box DNA-binding domain.
- Information by UniProt
- FLJ16340 antibody
- HBP 1 antibody
- HBP1 antibody
All lanes : Anti-HBP1 (phospho S402) antibody (ab73339) at 1/500 dilution
Lane 1 : Extracts from A549 cells treated with PMA (125ng/ml, 30 mins)
Lane 2 : Extracts from A549 cells treated with PMA (125ng/ml, 30 mins) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa
ab73339 has not yet been referenced specifically in any publications.