Anti-HBS1L antibody (ab217067)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-HBS1L antibody
See all HBS1L primary antibodies -
Description
Rabbit polyclonal to HBS1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human HBS1L aa 150-200. The exact sequence is proprietary.
Sequence:QTSRSESEIVP KVAKMTVSGK KQTMGFEVPG VSSEENGHSF HTPQKGPPIE
Database link: Q9Y450 -
Positive control
- HeLa, 293T and Jurkat whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab217067 was affinity purified using an epitope specific to HBS1L immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab217067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 75 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Tissue specificity
Detected in heart, brain, placenta, liver, muscle, kidney and pancreas. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. - Information by UniProt
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Database links
- Entrez Gene: 10767 Human
- Entrez Gene: 100173449 Orangutan
- Omim: 612450 Human
- SwissProt: Q9Y450 Human
- SwissProt: Q5R6Y0 Orangutan
- Unigene: 378532 Human
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Alternative names
- EF 1a antibody
- ERF3 similar protein antibody
- ERFS antibody
see all
Images
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Western blot - Anti-HBS1L antibody (ab217067)All lanes : Anti-HBS1L antibody (ab217067) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysates
Lane 2 : 293T whole cell lysates
Lane 3 : Jurkat whole cell lysates
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-HBS1L antibody (ab217067)Detection of HBS1L in immunoprecipitates of Jurkat whole cell lysate prepared using NETN lysis buffer (0.5 or 1 mg for IP, 20% of IP loaded) using ab217067 at 6 µg/mg lysate for IP (Lane 1), and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
Datasheets and documents
References
ab217067 has not yet been referenced specifically in any publications.
