Key features and details
- Rabbit polyclonal to HBS1L
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HBS1L antibody
See all HBS1L primary antibodies
DescriptionRabbit polyclonal to HBS1L
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- HeLa, 293T and Jurkat whole cell lysates.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab217067 was affinity purified using an epitope specific to HBS1L immobilized on solid support.
Our Abpromise guarantee covers the use of ab217067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 75 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Tissue specificityDetected in heart, brain, placenta, liver, muscle, kidney and pancreas.
Sequence similaritiesBelongs to the GTP-binding elongation factor family.
- Information by UniProt
- EF 1a antibody
- ERF3 similar protein antibody
- ERFS antibody
All lanes : Anti-HBS1L antibody (ab217067) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysates
Lane 2 : 293T whole cell lysates
Lane 3 : Jurkat whole cell lysates
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 10 seconds
Detection of HBS1L in immunoprecipitates of Jurkat whole cell lysate prepared using NETN lysis buffer (0.5 or 1 mg for IP, 20% of IP loaded) using ab217067 at 6 µg/mg lysate for IP (Lane 1), and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab217067 has not yet been referenced specifically in any publications.