Anti-HBS1L antibody (ab217067)
Key features and details
- Rabbit polyclonal to HBS1L
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HBS1L antibody
See all HBS1L primary antibodies -
Description
Rabbit polyclonal to HBS1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human HBS1L aa 150-200. The exact sequence is proprietary.
Sequence:QTSRSESEIVP KVAKMTVSGK KQTMGFEVPG VSSEENGHSF HTPQKGPPIE
Database link: Q9Y450 -
Positive control
- HeLa, 293T and Jurkat whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab217067 was affinity purified using an epitope specific to HBS1L immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab217067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 75 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Tissue specificity
Detected in heart, brain, placenta, liver, muscle, kidney and pancreas. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. - Information by UniProt
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Database links
- Entrez Gene: 10767 Human
- Entrez Gene: 100173449 Orangutan
- Omim: 612450 Human
- SwissProt: Q9Y450 Human
- SwissProt: Q5R6Y0 Orangutan
- Unigene: 378532 Human
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Alternative names
- EF 1a antibody
- ERF3 similar protein antibody
- ERFS antibody
see all
Images
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Western blot - Anti-HBS1L antibody (ab217067)All lanes : Anti-HBS1L antibody (ab217067) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysates
Lane 2 : 293T whole cell lysates
Lane 3 : Jurkat whole cell lysates
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-HBS1L antibody (ab217067)Detection of HBS1L in immunoprecipitates of Jurkat whole cell lysate prepared using NETN lysis buffer (0.5 or 1 mg for IP, 20% of IP loaded) using ab217067 at 6 µg/mg lysate for IP (Lane 1), and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
References (0)
ab217067 has not yet been referenced specifically in any publications.
