Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11531(B)] to HBXIP
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-HBXIP antibody [EPR11531(B)]
See all HBXIP primary antibodies
DescriptionRabbit monoclonal [EPR11531(B)] to HBXIP
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human HBXIP (UniProt O43504).
- BxPC-3 and MCF7 cell lysates; BxPC-3 cells; Immunoprecipitation pellet from BxPC-3 cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab157480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 10 kDa.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionWhen complexed to BIRC5, interferes with apoptosome assembly, preventing recruitment of pro-caspase-9 to oligomerized APAF1, thereby selectively suppressing apoptosis initiated via the mitochondrial/cytochrome c pathway. Down-regulates hepatitis B virus (HBV) replication.
Tissue specificityHighly expressed in skeletal and cardiac muscle, followed by pancreas, kidney, liver, brain, placenta and lung. Elevated levels in both cancerous and non-cancerous liver tissue of patients with chronic HBV infection compared with hepatic tissue without HBV infection.
Sequence similaritiesBelongs to the HBXIP family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- HBV X interacting protein antibody
- HBV X-interacting protein antibody
- HBX interacting protein antibody
All lanes : Anti-HBXIP antibody [EPR11531(B)] (ab157480) at 1/1000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 10 kDa
Flow cytometric analysis of permeabilized BxPC-3 cells labeling HBXIP with ab157480 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Anti-HBXIP antibody [EPR11531(B)] (ab157480) at 1/1000 dilution + immunoprecipitation pellet from BxPC-3 cell lysate
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 10 kDa
ab157480 has been referenced in 2 publications.
- Lei XY et al. Hepatitis B virus X protein decreases nephrin expression and induces podocyte apoptosis via activating STAT3. Exp Ther Med 17:4223-4229 (2019). PubMed: 31007753
- Liu F et al. The oncoprotein HBXIP enhances angiogenesis and growth of breast cancer through modulating FGF8 and VEGF. Carcinogenesis 35:1144-53 (2014). WB ; Human . PubMed: 24464787