Overview

  • Product name
    Anti-HBXIP antibody [EPR11531(B)]
    See all HBXIP primary antibodies
  • Description
    Rabbit monoclonal [EPR11531(B)] to HBXIP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human HBXIP (UniProt O43504).

  • Positive control
    • BxPC-3 and MCF7 cell lysates; BxPC-3 cells; Immunoprecipitation pellet from BxPC-3 cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 10 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      When complexed to BIRC5, interferes with apoptosome assembly, preventing recruitment of pro-caspase-9 to oligomerized APAF1, thereby selectively suppressing apoptosis initiated via the mitochondrial/cytochrome c pathway. Down-regulates hepatitis B virus (HBV) replication.
    • Tissue specificity
      Highly expressed in skeletal and cardiac muscle, followed by pancreas, kidney, liver, brain, placenta and lung. Elevated levels in both cancerous and non-cancerous liver tissue of patients with chronic HBV infection compared with hepatic tissue without HBV infection.
    • Sequence similarities
      Belongs to the HBXIP family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • HBV X interacting protein antibody
      • HBV X-interacting protein antibody
      • HBX interacting protein antibody
      • HBX-interacting protein antibody
      • hbxip antibody
      • HBXIP_HUMAN antibody
      • Hepatitis B virus X interacting protein antibody
      • Hepatitis B virus X-interacting protein antibody
      • LAMTOR5 antibody
      • Late endosomal/lysosomal adaptor MAPK and MTOR activator 5 antibody
      • MGC71071 antibody
      • Ragulator complex protein LAMTOR5 antibody
      • XIP antibody
      see all

    Images

    • All lanes : Anti-HBXIP antibody [EPR11531(B)] (ab157480) at 1/1000 dilution

      Lane 1 : BxPC-3 cell lysate
      Lane 2 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 10 kDa

    • Flow cytometric analysis of permeabilized BxPC-3 cells labeling HBXIP with ab157480 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
    • Anti-HBXIP antibody [EPR11531(B)] (ab157480) at 1/1000 dilution + immunoprecipitation pellet from BxPC-3 cell lysate

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 10 kDa

    References

    This product has been referenced in:
    • Liu F  et al. The oncoprotein HBXIP enhances angiogenesis and growth of breast cancer through modulating FGF8 and VEGF. Carcinogenesis 35:1144-53 (2014). WB ; Human . Read more (PubMed: 24464787) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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