Product nameAnti-HBZ antibody
See all HBZ primary antibodies
DescriptionRabbit polyclonal to HBZ
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Cow, Pig, Chimpanzee
- K562 cell lysate. Human liver.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab62266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
FunctionThe zeta chain is an alpha-type chain of mammalian embryonic hemoglobin, synthesized primarily in the yolk sac.
Tissue specificityRed blood cells.
Sequence similaritiesBelongs to the globin family.
- Information by UniProt
- HBAZ antibody
- HBAZ_HUMAN antibody
- HBZ 2 antibody
All lanes : HBZ antibody (ab62266) at 1.25µg/ml in 5% skim milk / PBS buffer
Lane 1 : Molecular weight marker
Lane 2 : K562 cell lysate at 10 µg
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 15%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelled HBZ with ab62266 at 4-8µg/ml. Arrows indicate positively labelled hemopoietic cells. Magnification: 400X.
ab62266 has not yet been referenced specifically in any publications.