Anti-HC-II antibody (ab196758)
Key features and details
- Rabbit polyclonal to HC-II
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HC-II antibody
See all HC-II primary antibodies -
Description
Rabbit polyclonal to HC-II -
Host species
Rabbit -
Specificity
The antibody detects endogenous levels of total Heparin Cofactor II protein.
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Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human HC-II (internal sequence).
Database link: P05546 -
Positive control
- WB: K562 whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab196758 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
Target
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Function
Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils. -
Tissue specificity
Expressed predominantly in liver. Also present in plasma. -
Involvement in disease
Thrombophilia due to heparin cofactor 2 deficiency -
Sequence similarities
Belongs to the serpin family. -
Domain
The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition. -
Post-translational
modificationsPhosphorylation sites are present in the extracellular medium. - Information by UniProt
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Database links
- Entrez Gene: 3053 Human
- Omim: 142360 Human
- SwissProt: P05546 Human
- Unigene: 474270 Human
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Alternative names
- D22S673 antibody
- HC II antibody
- HC-II antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196758 has not yet been referenced specifically in any publications.