Key features and details
- Rabbit polyclonal to HC-II
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-HC-II antibody
See all HC-II primary antibodies
DescriptionRabbit polyclonal to HC-II
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Rat, Rabbit
Recombinant fragment (His-T7-tag) corresponding to Human HC-II aa 276-499. Expressed in E. coli. N-terminal tags.
ALENIDPATQMMILNCIYFKGSWVNKFPVEMTHNHNFRLNEREVVKVSMM QTKGNFLAANDQELDCDILQLEYVGGISMLIVVPHKMSGMKTLEAQLTPR VVERWQKSMTNRTREVLLPKFKLEKNYNLVESLKLMGIRMLFDKNGNMAG ISDQRIAIDLFKHQGTITVNEEGTQATTVTTVGFMPLSTQVRFTVDRPFL FLIYEHRTSCLLFMGRVANPSRS
Database link: P05546
- IHC-P: Human liver tissue. WB: Recombinant human HC-II protein. Pig lung, liver and kidney tissue lysate. Human serum.
Protein previously labeled as Heparin Cofactor II.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionThrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.
Tissue specificityExpressed predominantly in liver. Also present in plasma.
Involvement in diseaseThrombophilia due to heparin cofactor 2 deficiency
Sequence similaritiesBelongs to the serpin family.
DomainThe N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition.
modificationsPhosphorylation sites are present in the extracellular medium.
- Information by UniProt
- D22S673 antibody
- HC II antibody
- HC-II antibody
All lanes : Anti-HC-II antibody (ab232859) at 1 µg/ml
Lane 1 : Human serum
Lane 2 : Pig lung tissue lysate
Lane 3 : Pig liver tissue lysate
Lane 4 : Pig kidney tissue lysate
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/1000 dilution
Formalin-fixed, paraffin-embedded human liver tissue stained for HC-II with ab232859 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver tissue stained for HC-II with ab232859 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Anti-HC-II antibody (ab232859) at 2 µg/ml + Recombinant human HC-II protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232859 has not yet been referenced specifically in any publications.