Overview

  • Product name

  • Description

    Rabbit polyclonal to hCAP-D3
  • Host species

    Rabbit
  • Specificity

    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between C terminal residues 1450-1507 of human hCAP-D3. (NP_056076.1)

  • Positive control

    • HeLa and 293T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 1 to 4 µg/mg of lysate
    WB: 1/2000 - 1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 168 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis.
    • Sequence similarities

      Contains 4 HEAT repeats.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • CAP D3 antibody
      • CAPD 3 antibody
      • CAPD3 antibody
      • CNDD3_HUMAN antibody
      • Condensin II complex subunit D3 antibody
      • Condensin-2 complex subunit D3 antibody
      • FLJ42888 antibody
      • hCAP-D3 antibody
      • Hcp 6 antibody
      • Hcp6 antibody
      • hHCP 6 antibody
      • hHCP6 antibody
      • KIAA0056 antibody
      • MGC104671 antibody
      • NCAPD 3 antibody
      • Ncapd3 antibody
      • Non SMC condensin II complex subunit D3 antibody
      • Non-SMC condensin II complex subunit D3 antibody
      • OTTHUMP00000235403 antibody
      • OTTHUMP00000235404 antibody
      see all

    Images

    • All lanes:200µg Hela whole cell lysate and ab70349 at 1µg/ml. Lane 1: ab70349 at 3 µg/mg lysate for IP. Lane 2: IgG control.
    • All lanes : Anti-hCAP-D3 antibody (ab70349) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size: 168 kDa
      Observed band size: 174 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Wallace HA  et al. Condensin II subunit NCAPH2 associates with shelterin protein TRF1 and is required for telomere stability. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 31026066) »
    • Yokoyama Y  et al. A novel role for the condensin II complex in cellular senescence. Cell Cycle 14:2160-70 (2015). Read more (PubMed: 26017022) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (fibroblast)
    Loading amount
    50 µg
    Specification
    fibroblast
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted May 10 2013

    Question
    Answer

    Thank you for contacting us.
    As discussed please let me know if you are interested in the 100% Abreview testing discount programme.
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