Anti-hCAP-D3 antibody (ab70349)
Key features and details
- Rabbit polyclonal to hCAP-D3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-hCAP-D3 antibody
See all hCAP-D3 primary antibodies -
Description
Rabbit polyclonal to hCAP-D3 -
Host species
Rabbit -
Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to a region between C terminal residues 1450-1507 of human hCAP-D3. (NP_056076.1)
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Positive control
- HeLa and 293T whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
IP |
Notes |
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WB: 1/2000 - 1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 168 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis. -
Sequence similarities
Contains 4 HEAT repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23310 Human
- Omim: 609276 Human
- SwissProt: P42695 Human
- Unigene: 438550 Human
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Alternative names
- CAP D3 antibody
- CAPD 3 antibody
- CAPD3 antibody
see all
Images
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All lanes:200µg Hela whole cell lysate and ab70349 at 1µg/ml. Lane 1: ab70349 at 3 µg/mg lysate for IP. Lane 2: IgG control.
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All lanes : Anti-hCAP-D3 antibody (ab70349) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 168 kDa
Observed band size: 174 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab70349 has been referenced in 9 publications.
- Choi EH et al. Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes. Genome Biol 23:70 (2022). PubMed: 35241136
- Winans S et al. A point mutation in HIV-1 integrase redirects proviral integration into centromeric repeats. Nat Commun 13:1474 (2022). PubMed: 35304442
- Wallace HA et al. Condensin II subunit NCAPH2 associates with shelterin protein TRF1 and is required for telomere stability. J Cell Physiol N/A:N/A (2019). PubMed: 31026066
- Baergen AK et al. Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis. Cancers (Basel) 11:N/A (2019). PubMed: 31357676
- Poser E et al. Aurora A promotes chromosome congression by activating the condensin-dependent pool of KIF4A. J Cell Biol 219:N/A (2019). PubMed: 31881080
- Abramo K et al. A chromosome folding intermediate at the condensin-to-cohesin transition during telophase. Nat Cell Biol 21:1393-1402 (2019). PubMed: 31685986
- Yokoyama Y et al. A novel role for the condensin II complex in cellular senescence. Cell Cycle 14:2160-70 (2015). PubMed: 26017022
- Liu W et al. PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature 466:508-12 (2010). WB ; Human . PubMed: 20622854
- Centore RC et al. CRL4(Cdt2)-mediated destruction of the histone methyltransferase Set8 prevents premature chromatin compaction in S phase. Mol Cell 40:22-33 (2010). WB ; Human . PubMed: 20932472