Product nameAnti-HCC1 antibody
See all HCC1 primary antibodies
DescriptionRabbit polyclonal to HCC1
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human HCC1 aa 325-375. The exact sequence is proprietary.
Database link: Q14498
EpitopeThe epitope recognized by this antibody maps to a region between residues 325 and 375 of human HCC1.
- IHC-P: Human prostate carcinoma tissue. WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HCC1 in HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab25801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 60 kDa).|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionTranscriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1 (By similarity). May be involved in pre-mRNA splicing process.
Tissue specificityWidely expressed. Highly expressed in pancreas, skeletal muscle, lung and brain. Expressed at intermediate level in kidney, liver and heart.
Sequence similaritiesBelongs to the splicing factor SR family.
Contains 3 RRM (RNA recognition motif) domains.
Cellular localizationNucleus speckle. Concentrated in nuclear speckles. Colocalizes with the core spliceosomal snRNP proteins.
- Information by UniProt
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All lanes : Anti-HCC1 antibody (ab25801) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 60 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for HCC1 with ab25801 at a 1/5000 dilution in immunohistochemical analysis. DAB staining.
HCC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer, with ab25801 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab25801 at 1 µg/ml.
Lane 1: ab25801 used for IP at 6 µg per reaction (IP by a previous lot of this antibody)
Lane 2: ab25801 used for IP at 6 µg per reaction
Lane 3: Control IgG.
Chemiluminescence detection: 30 seconds.
ab25801 has not yet been referenced specifically in any publications.