Anti-HCCA2 antibody (ab115013)
Key features and details
- Goat polyclonal to HCCA2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-HCCA2 antibody -
Description
Goat polyclonal to HCCA2 -
Host species
Goat -
Specificity
ab115013 is expected to recognize both reported isoforms (NP_001165694.1; NP_443731.2) only. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog -
Immunogen
Synthetic peptide:
DERGKKVKCTAPQ
, corresponding to internal sequence amino acids 128-140 of Human HCCA2 (NP_001165694.1; NP_443731.2). -
Positive control
- Jurkat cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab115013 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.02 - 0.06 µg/ml. Detects a band of approximately 30, 48 kDa (predicted molecular weight: 30 kDa).
A 1 hour primary incubation is recommended for this product. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide. |
Notes |
---|
IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.02 - 0.06 µg/ml. Detects a band of approximately 30, 48 kDa (predicted molecular weight: 30 kDa). A 1 hour primary incubation is recommended for this product. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide. |
Target
-
Function
Stimulates the autophosphorylation and kinase activity of STK38 and STK38L. -
Sequence similarities
Belongs to the MOB1/phocein family. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Nucleus. Cytoplasm > perinuclear region. - Information by UniProt
-
Database links
- Entrez Gene: 81532 Human
- Entrez Gene: 101513 Mouse
- Entrez Gene: 499288 Rat
- Omim: 611969 Human
- SwissProt: Q70IA6 Human
- SwissProt: Q8VI63 Mouse
- Unigene: 370360 Human
- Unigene: 53259 Mouse
-
Alternative names
- HCCA2 antibody
- HCCA2 gene antibody
- HCCA2 protein antibody
see all
Images
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab115013 has not yet been referenced specifically in any publications.