Product nameAnti-HCE antibody
See all HCE primary antibodies
DescriptionRabbit polyclonal to HCE
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- HeLa, 293T and NIH3T3 cell lysates, Human ovarian carcinoma tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 69 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/250.|
FunctionBifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
Tissue specificityIsoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle.
Sequence similaritiesIn the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.
In the C-terminal section; belongs to the eukaryotic GTase family.
- Information by UniProt
- CAP1A antibody
- GTase antibody
- GTP--RNA guanylyltransferase antibody
All lanes : Anti-HCE antibody (ab176728) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 3 minutes
Immunohistochemical analysis of formalin fixed, paraffin embedded Human ovarian carcinoma tissue labeling HCE using ab176728 at a 1/200 dilution (1µg/ml), using peroxidase substrate for detection.
Western blot of immunopricipitate of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), labeling HCE using ab176728 for WB at 0.4 µg/ml and used for IP at 3 µg/mg lysate.
ab176728 has not yet been referenced specifically in any publications.