Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal (ab137618)
Key features and details
- Rabbit polyclonal to HCF-1 / Host Cell Factor C1 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal
See all HCF-1 / Host Cell Factor C1 primary antibodies -
Description
Rabbit polyclonal to HCF-1 / Host Cell Factor C1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 49-269 of Human HCF-1/ Host Cell Factor C1.
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Positive control
- A549, HeLa, HepG2 cell lysates; Mouse brain; HeLa cells; BT474 xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 208 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 208 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes. -
Tissue specificity
Highly expressed in fetal tissues and the adult kidney. Present in all tissues tested. -
Sequence similarities
Contains 5 Kelch repeats. -
Domain
The HCF repeat is a highly specific proteolytic cleavage signal.
The kelch repeats fold into a 6-bladed kelch beta-propeller called the beta-propeller domain which mediates interaction with HCFC1R1. -
Post-translational
modificationsProteolytically cleaved at one or several PPCE--THET sites within the HCF repeats. Further cleavage of the primary N- and C-terminal chains results in a 'trimming' and accumulation of the smaller chains.
O-glycosylated.
Ubiquitinated. Lys-1807 and Lys-1808 are ubiquitinated both via 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. BAP1 mediated deubiquitination of 'Lys-48'-linked polyubiquitin chains; deubiquitination by BAP1 does not seem to stabilize the protein. -
Cellular localization
Cytoplasm. Nucleus. HCFC1R1 modulates its subcellular localization and overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm. Nuclear in general, but uniquely cytoplasmic in trigeminal ganglia, becoming nuclear upon HSV reactivation from the latent state. - Information by UniProt
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Database links
- Entrez Gene: 3054 Human
- Entrez Gene: 15161 Mouse
- Entrez Gene: 363519 Rat
- Omim: 300019 Human
- SwissProt: P51610 Human
- SwissProt: Q61191 Mouse
- Unigene: 83634 Human
- Unigene: 248353 Mouse
see all -
Alternative names
- C1 factor antibody
- CFF antibody
- HCF 1 antibody
see all
Images
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Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal (ab137618) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 208 kDa
5% SDS PAGE -
Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal (ab137618) at 1/500 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 208 kDa
5% SDS PAGE -
ab137618 at 1/200 dilution staining HCF-1 / Host Cell Factor C1 in methanol fixed HeLa cells by Immunofluorescence. Lower image costained with Hoerchst 33342.
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ab137618 at 1/100 dilution, staining HCF-1 / Host Cell Factor C1 in paraffin embedded BT474 xenograft tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137618 has not yet been referenced specifically in any publications.