Overview

  • Product name

    Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal
  • Description

    Rabbit polyclonal to HCF-1 / Host Cell Factor C1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 49-269 of Human HCF-1/ Host Cell Factor C1.

  • Positive control

    • A549, HeLa, HepG2 cell lysates; Mouse brain; HeLa cells; BT474 xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab137618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 208 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2; represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription, respectively) together. In case of human herpes simplex virus (HSV) infection, HCFC1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and POU2F1 thereby enabling the transcription of the viral immediate early genes.
  • Tissue specificity

    Highly expressed in fetal tissues and the adult kidney. Present in all tissues tested.
  • Sequence similarities

    Contains 5 Kelch repeats.
  • Domain

    The HCF repeat is a highly specific proteolytic cleavage signal.
    The kelch repeats fold into a 6-bladed kelch beta-propeller called the beta-propeller domain which mediates interaction with HCFC1R1.
  • Post-translational
    modifications

    Proteolytically cleaved at one or several PPCE--THET sites within the HCF repeats. Further cleavage of the primary N- and C-terminal chains results in a 'trimming' and accumulation of the smaller chains.
    O-glycosylated.
    Ubiquitinated. Lys-1807 and Lys-1808 are ubiquitinated both via 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. BAP1 mediated deubiquitination of 'Lys-48'-linked polyubiquitin chains; deubiquitination by BAP1 does not seem to stabilize the protein.
  • Cellular localization

    Cytoplasm. Nucleus. HCFC1R1 modulates its subcellular localization and overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm. Nuclear in general, but uniquely cytoplasmic in trigeminal ganglia, becoming nuclear upon HSV reactivation from the latent state.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1 factor antibody
    • CFF antibody
    • HCF 1 antibody
    • HCF antibody
    • HCF C-terminal chain 6 antibody
    • HCF-1 antibody
    • HCF1 antibody
    • Hcfc1 antibody
    • HCFC1_HUMAN antibody
    • HFC1 antibody
    • Host cell factor 1 antibody
    • Host cell factor antibody
    • Host cell factor C1 (VP16 accessory protein) antibody
    • Host cell factor C1 antibody
    • MGC70925 antibody
    • MRX3 antibody
    • VCAF antibody
    • VP16 accessory protein antibody
    see all

Images

  • Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal (ab137618) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 208 kDa



    5% SDS PAGE
  • Anti-HCF-1 / Host Cell Factor C1 antibody - N-terminal (ab137618) at 1/500 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 208 kDa



    5% SDS PAGE
  • ab137618 at 1/200 dilution staining HCF-1 / Host Cell Factor C1 in methanol fixed HeLa cells by Immunofluorescence. Lower image costained with Hoerchst 33342.
  • ab137618 at 1/100 dilution, staining HCF-1 / Host Cell Factor C1 in paraffin embedded BT474 xenograft tissue by Immunohistochemistry.

References

ab137618 has not yet been referenced specifically in any publications.

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