Overview

  • Product name
    Anti-hCG alpha 6 antibody [INN-hCG-72]
  • Description
    Mouse monoclonal [INN-hCG-72] to hCG alpha 6
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, RIA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length hCG (Human).

  • Epitope
    Alpha 6 epitope on hCG alpha.

Properties

Applications

Our Abpromise guarantee covers the use of ab11387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
RIA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 20-28 kDa.

Target

  • Relevance
    The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The alpha subunit belongs to the glycoprotein hormones alpha chain family.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • CG alpha antibody
    • CGA antibody
    • FSH alpha antibody
    • Glycoprotein hormones alpha chain antibody
    • LSH alpha antibody
    • TSH alpha antibody
    see all

References

ab11387 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your reply and for your question.

The lab has found that the volume is critical to the reaction, and theefficiency of the reaction will be significantly lower if more than 100 uL used. With 200 uL, I would be concerned that very little antibody will be conjugated.

I hope this will be helpful, but please let me know if you have further questions and I'll be happy to help.

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Answer

Thank you for contacting us with your question.

We do have abiotinylation kit thatis suitable to use with these detection antibodies, ab102864-

https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100µg-Biotin-ab102864.html

For this kit, the minimum starting concentration is 1 mg/mL, and azide up to 0.1% has negligible effect. The kit contains 3 vials that can each conjugate 100 uL of antibody, so you would need a couple of kits in order to conjugate the entire contents of ab11232 and ab11386 (250 uL each).

Would this kit work for you? Please let me know if you have further questions or if there is anything else that we can do for you, and I'll be happy to help.

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Answer

Thank you for your enquiry. For ab11387, the half-life is not known. In addition I would like to refer you to the following publication for information on the molecular weight. It appears to be about 23kDa. The citation details are as follows: Berger P et al (1993) J. Clin. Endocrinol. Metab. 77(2):347-351. For ab1107, the molecular weight is approximately 150 kDa and the half-life is unknown. Regarding both, we don't have any information about whether they could go through a humanisation process.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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