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    hcg-antibody-ab9376.pdf

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Signal Transduction Growth Factors/Hormones Hormones
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Anti-hCG antibody (ab9376)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG antibody (ab9376)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG antibody (ab9376)

Key features and details

  • Rabbit polyclonal to hCG
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-hCG antibody
    See all hCG primary antibodies
  • Description

    Rabbit polyclonal to hCG
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with hCG. It stains strongly with the human placenta and placental trophoblasts. No non-specific stainingwith any other tissue has been observed.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Beta chain of human chorionic gonadotropin.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Liquid antiserum
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Cancer
    • Growth factors
    • Hormones
    • Cancer
    • Tumor biomarkers
    • Hormones
    • Developmental Biology
    • Reproduction
    • Hormones
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab9376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC: This antibody may be diluted to a titer of 1:100-1:500 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
    Although not required, proteolytic treatment may enhance the immunostaining in overfixed tissues.
  • Target

    • Relevance

      Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.
    • Cellular localization

      Secreted
    • Database links

      • Entrez Gene: 1081 Human
      • Entrez Gene: 1082 Human
      • Omim: 118850 Human
      • Omim: 118860 Human
      • SwissProt: P01215 Human
      • SwissProt: P01233 Human
      • Unigene: 119689 Human
      • Unigene: 446683 Human
      • Unigene: 743279 Human
      see all
    • Alternative names

      • anterior pituitary glycoprotein hormones common subunit alpha antibody
      • CG alpha antibody
      • CG-ALPHA antibody
      • CGB antibody
      • CGB3 antibody
      • CGB5 antibody
      • CGB7 antibody
      • choriogonadotropin alpha chain antibody
      • Chorionic gonadotrophin chain beta antibody
      • chorionic gonadotrophin subunit alpha antibody
      • chorionic gonadotropin beta 3 subunit antibody
      • Chorionic gonadotropin beta chain antibody
      • Chorionic gonadotropin beta subunit antibody
      • chorionic gonadotropin, alpha polypeptide antibody
      • chorionic gonadotropin, beta polypeptide 3 antibody
      • chorionic gonadotropin, beta polypeptide antibody
      • Follicle stimulating hormone alpha chain antibody
      • follicle-stimulating hormone alpha chain antibody
      • follicle-stimulating hormone alpha subunit antibody
      • follitropin alpha chain antibody
      • FSH Alpha antibody
      • FSH-alpha antibody
      • FSHA antibody
      • glycoprotein hormones alpha chain antibody
      • glycoprotein hormones, alpha polypeptide antibody
      • GPHa antibody
      • GPHA1 antibody
      • HCG antibody
      • hCGB antibody
      • Human Chorionic Gonadotropin antibody
      • LHA antibody
      • LSH alpha antibody
      • LSH-alpha antibody
      • luteinizing hormone alpha chain antibody
      • lutropin alpha chain antibody
      • Thyroid stimulating hormone alpha chain antibody
      • thyroid-stimulating hormone alpha chain antibody
      • thyrotropin alpha chain antibody
      • TSH Alpha antibody
      • TSH-alpha antibody
      • TSHA antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG antibody (ab9376)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG antibody (ab9376)
      Human normal placenta. Staining is localised extracellular and in the cytoplasm. Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG antibody (ab9376)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG antibody (ab9376)
      ab9376 staining hCG in formalin-fixed, paraffin-embedded Human placenta by Immunohistochemistry.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab9376? Please let us know so that we can cite the reference in this datasheet.

    ab9376 has been referenced in 5 publications.

    • Kojima J  et al. Efficient production of trophoblast lineage cells from human induced pluripotent stem cells. Lab Invest 97:1188-1200 (2017). PubMed: 28287635
    • Peng B  et al. Gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor are expressed at tubal ectopic pregnancy implantation sites. Fertil Steril 105:1620-1627.e3 (2016). PubMed: 26920257
    • Wang JM  et al. The human leukocyte antigen G promotes trophoblast fusion and ß-hCG production through the Erk1/2 pathway in human choriocarcinoma cell lines. Biochem Biophys Res Commun 434:460-5 (2013). PubMed: 23583402
    • Dukic-Stefanovic S  et al. Chorionic gonadotropin and its receptor are both expressed in human retina, possible implications in normal and pathological conditions. PLoS One 7:e52567 (2012). IHC-Fr ; Human . PubMed: 23285091
    • Kane N  et al. Proliferation of uterine natural killer cells is induced by human chorionic gonadotropin and mediated via the mannose receptor. Endocrinology 150:2882-8 (2009). ICC/IF ; Human . PubMed: 19196802

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-hCG antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (trophoblast)
    Loading amount
    10 µg
    Specification
    trophoblast
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    0.5%Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Kai Wang

    Verified customer

    Submitted Apr 19 2012

    Question

    Testing discount code to try in WB.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 27 2012

    Answer

    DISCOUNT CODE: ********
    Expiration date: **********

    I am very pleased to hear you would like to accept our offer and test ab9376 in western blot. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for western blot and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Mar 27 2012

    Question

    we are intersted to know the avilablity of the antibodies clubbed with some matrix for use in affinity chromatographic purification of HCG.pls send quote if available.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 17 2002

    Answer

    Dear Sir/Madam I am afraid that this antibody is not available in a form conjugated to a matrix. However, I believe Mobitec and Amersham Biosciences may be able to supply you with the matrices to conjugate our antibody to. I believe the procedures required are quite simple. I am sorry I can't be of any more assistance. I hope this helps. Regards Michael

    Read More

    Michael Chipchase

    Abcam Scientific Support

    Answered on Apr 18 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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