Key features and details
- Mouse monoclonal [HCG1] to hCG
- Suitable for: ELISA, RIA, RID
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-hCG antibody [HCG1]
See all hCG primary antibodies
DescriptionMouse monoclonal [HCG1] to hCG
SpecificityhCG specifically recognises human chorionic gonadotropin. Its affinity for the free beta-chain is slightly greater than its affinity for the whole molecule. hCG - 100% hCG (beta) - 125% hCG (alpha) - <1% hLH - <1% hTSH - <0.05% hFSH - <<0.1% Affinity constant: 5x109 l/mol HCG.
Tested applicationsSuitable for: ELISA, RIA, RIDmore details
Species reactivityReacts with: Human
General notesAssociation Constant: hCG: 4.0 x 106 and hCGbeta: 1.6 x 106
Dissociation Constant: hCG: 1.4 x 10-4 and hCGbeta: 3.6 x 10-4
Affinity Constant hCG: KA = 2.9 x 1010 1/M; KD = 3.4 x 10-11 M (= 0.03 nM)
Affinity Constant hCGbeta: KA = 4.4 x 109 1/M; KD = 2.3 x 10-10 M (= 0.23 nM)
Determination method: SPR analysis
Antigen: hCG; hCG-beta
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 0.9% Sodium chloride
Concentration information loading...
PurityProtein A purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|RID||Use at an assay dependent dilution.|
RelevanceHuman chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.
- anterior pituitary glycoprotein hormones common subunit alpha antibody
- CG alpha antibody
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab400 has been referenced in 5 publications.
- Haslam C et al. Label-Free Sensors Based on Graphene Field-Effect Transistors for the Detection of Human Chorionic Gonadotropin Cancer Risk Biomarker. Diagnostics (Basel) 8:N/A (2018). PubMed: 29316718
- Xu RH et al. BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol 20:1261-4 (2002). PubMed: 12426580
- Birnbaum S et al. Latex-based thin-layer immunoaffinity chromatography for quantitation of protein analytes. Anal Biochem 206:168-71 (1992). PubMed: 1456429
- Pettersson K et al. Time-resolved fluoroimmunoassay of human choriogonadotropin. Clin Chem 29:60-4 (1983). PubMed: 6848283
- Wahlstrom T et al. The use of monoclonal antibodies against human chorionic gonadotropin for immunoperoxidase staining of normal placenta, pituitary gland, and pituitary adenomas. J Histochem Cytochem 29:864-5 (1981). PubMed: 6167612