• Product name
    Anti-hCG antibody [HCG1]
    See all hCG primary antibodies
  • Description
    Mouse monoclonal [HCG1] to hCG
  • Host species
  • Specificity
    hCG specifically recognises human chorionic gonadotropin. Its affinity for the free beta-chain is slightly greater than its affinity for the whole molecule. hCG - 100% hCG (beta) - 125% hCG (alpha) - <1% hLH - <1% hTSH - <0.05% hFSH - <<0.1% Affinity constant: 5x109 l/mol HCG.
  • Tested applications
    Suitable for: ELISA, RIA, RIDmore details
  • Species reactivity
    Reacts with: Human
  • General notes
    Association Constant: hCG: 4.0 x 106 and hCGbeta: 1.6 x 106
    Dissociation Constant: hCG: 1.4 x 10-4 and hCGbeta: 3.6 x 10-4
    Affinity Constant hCG: KA = 2.9 x 1010 1/M; KD = 3.4 x 10-11 M (= 0.03 nM)
    Affinity Constant hCGbeta: KA = 4.4 x 109 1/M; KD = 2.3 x 10-10 M (= 0.23 nM)
    Determination method: SPR analysis
    Antigen: hCG; hCG-beta



Our Abpromise guarantee covers the use of ab400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
RID Use at an assay dependent dilution.


  • Relevance
    Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.
  • Cellular localization
  • Database links
  • Alternative names
    • anterior pituitary glycoprotein hormones common subunit alpha antibody
    • CG alpha antibody
    • CG-ALPHA antibody
    • CGB antibody
    • CGB3 antibody
    • CGB5 antibody
    • CGB7 antibody
    • choriogonadotropin alpha chain antibody
    • Chorionic gonadotrophin chain beta antibody
    • chorionic gonadotrophin subunit alpha antibody
    • chorionic gonadotropin beta 3 subunit antibody
    • Chorionic gonadotropin beta chain antibody
    • Chorionic gonadotropin beta subunit antibody
    • chorionic gonadotropin, alpha polypeptide antibody
    • chorionic gonadotropin, beta polypeptide 3 antibody
    • chorionic gonadotropin, beta polypeptide antibody
    • Follicle stimulating hormone alpha chain antibody
    • follicle-stimulating hormone alpha chain antibody
    • follicle-stimulating hormone alpha subunit antibody
    • follitropin alpha chain antibody
    • FSH Alpha antibody
    • FSH-alpha antibody
    • FSHA antibody
    • glycoprotein hormones alpha chain antibody
    • glycoprotein hormones, alpha polypeptide antibody
    • GPHa antibody
    • GPHA1 antibody
    • HCG antibody
    • hCGB antibody
    • Human Chorionic Gonadotropin antibody
    • LHA antibody
    • LSH alpha antibody
    • LSH-alpha antibody
    • luteinizing hormone alpha chain antibody
    • lutropin alpha chain antibody
    • Thyroid stimulating hormone alpha chain antibody
    • thyroid-stimulating hormone alpha chain antibody
    • thyrotropin alpha chain antibody
    • TSH Alpha antibody
    • TSH-alpha antibody
    • TSHA antibody
    see all


This product has been referenced in:
  • Haslam C  et al. Label-Free Sensors Based on Graphene Field-Effect Transistors for the Detection of Human Chorionic Gonadotropin Cancer Risk Biomarker. Diagnostics (Basel) 8:N/A (2018). Read more (PubMed: 29316718) »
  • Xu RH  et al. BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol 20:1261-4 (2002). Read more (PubMed: 12426580) »

See all 5 Publications for this product

Customer reviews and Q&As

This clone has been reported to detect intact hCG, hyperglycosylated hCG, nicked hCG, hCGb and nicked hCGb.

The antibody ab400 was tested for specific binding to hCG, but not the hCG we supply as ab126653. There is no published journal reference for the testing of the antibody ab400, and I am fairly certain there is no commercial source of the hCG protein th...

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We have not tested the two products together, ab400 antibody and ab126653 protein, but we assume that ab126653 is almost identical, if not exactly identical, to the hCG beta that was used to determine the binding constants listed in the general notes s...

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Thank you for your reply.

Just one more thing for clarification:

With "mouse", do you mean it should be mouse monoclonal or against the species mouse?

Sorry for the delay.

Thank you for contacting us.

Unfortunately, we do not sell this antibody in the conjugated form nor do we offer a conjugation service. However, we do have a conjugation kit available.

ab102891 EasyLink HRP Conjugation Kit (3 x 100µg ...

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Thank you for your patience.

Sorry for the late response. The specific pI range of ab400 is 6.4-7.9. However, we are sorry that we have not determined the pH of the storage buffer.

I hope this information is helpful to you. Please...

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Thank you for contacting us.

AB400 is shipped in a liquid form in which the antibody is suspended in a solution containing 0.9% Sodium Chloride and0.1% Sodium Azide (as a preservative).

AB9071is shipped in ...

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Thank you for contacting us and sorry for the delay of our reply. The source of the anti-hCG ab400 sent us the following kinetic parameters for this product : Association constants : whole hCG : 4.0 x 10^6 hCG (beta-subunit) : 1.6 x 10^6 D...

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Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Human Purified protein (Pregnancy hCG)
Loading amount
1000 µg
Pregnancy hCG
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 21 2007

Thank you for your enquiry and patience. I regret to inform you that this antibody has not been tested for application in IF and I have updated the online datasheet to reflect this. If you have any additional questions, please contact us again.

1-10 of 11 Abreviews or Q&A


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