Anti-hCG antibody [RM330] (ab238319)
![Western blot - Anti-hCG antibody [RM330] (ab238319)](https://www.abcam.com/ps/products/238/ab238319/Images/ab238319-316137-19b.jpg "Western blot - Anti-hCG antibody [RM330] (ab238319)")
Key features and details
- Rabbit monoclonal [RM330] to hCG
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-hCG antibody [RM330]
See all hCG primary antibodies -
Description
Rabbit monoclonal [RM330] to hCG -
Host species
Rabbit -
Specificity
ab238319 reacts to hCG. The antibody recognizes both the native complex and the ß subunit of hCG. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human hCG. (Purified from human urine)
Database link: P01215 -
Positive control
- WB: hGC purified from human urine. IHC-P: Human placenta tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM330 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab238319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. | |
| IHC-P | 1/500 - 1/1000. |
Target
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Relevance
Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 1081 Human
- Entrez Gene: 1082 Human
- Omim: 118850 Human
- Omim: 118860 Human
- SwissProt: P01215 Human
- SwissProt: P01233 Human
- Unigene: 119689 Human
- Unigene: 446683 Human
see all -
Alternative names
- anterior pituitary glycoprotein hormones common subunit alpha antibody
- CG alpha antibody
- CG-ALPHA antibody
see all
Images
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Western blot - Anti-hCG antibody [RM330] (ab238319)Anti-hCG antibody [RM330] (ab238319) at 1/20000 dilution + Native hGC purified from human urine
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG antibody [RM330] (ab238319)](https://www.abcam.com/ps/products/238/ab238319/Images/ab238319-316136-19a.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG antibody [RM330] (ab238319)Formalin-fixed, paraffin-embedded human placenta tissue stained for hGC using ab238319 at 1/1000 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab238319 has not yet been referenced specifically in any publications.
