Anti-hCG beta antibody [5H4-E2] (ab9582)
Key features and details
- Mouse monoclonal [5H4-E2] to hCG beta
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-hCG beta antibody [5H4-E2]
See all hCG beta primary antibodies -
Description
Mouse monoclonal [5H4-E2] to hCG beta -
Host species
Mouse -
Specificity
The antibody recognizes the whole hCG beta and the C-terminal end part of hCG beta, as well as the hCG dimer molecule. It does not react with hCG alpha, FSH, LH, TSH and albumins (evaluated by simple-binding ELISA using 1.00 µg/well purified antigens (WHO standards) and 1.00 µg/ml primary antibody concentrations). This antibody labels the cytoplasm of hCG beta producing (trophoblastic) cells in placenta, mola hydatidosa, choriocarcinoma, embryonal carcinoma and some undifferenciated lung cancers, as well as, the JAR choriocarcinoma cell line. It does not react with cells of normal endometrial, testicular tissues and lung (tested by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections using the Streptavidin-biotinylated peroxidase method). -
Tested Applications & Species
Application Species IHC-P Human -
Immunogen
Full length native protein (purified) corresponding to Human hCG beta.
Database link: P01233 -
Positive control
- ICC/IF: Human chorionic villus cells. IHC-P: Human fetal chorionic villus tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.097% Sodium azide
Constituents: Tris HCl, 0.2% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5H4-E2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. It gives optimal immunostaining with the majority of well-known detection systems (e.g. LSAB, ABC/SABC, PAP, APAAP, double staining, etc.). Antigen retrieval, such as heating of slides in 10 mM citrate buffer, pH 6.00 or proteolytic pre-treatment of tissues may increase the staining intensity. The recommended time of incubation is 60 minutes at room temperature.
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Notes |
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IHC-P
Use at an assay dependent concentration. It gives optimal immunostaining with the majority of well-known detection systems (e.g. LSAB, ABC/SABC, PAP, APAAP, double staining, etc.). Antigen retrieval, such as heating of slides in 10 mM citrate buffer, pH 6.00 or proteolytic pre-treatment of tissues may increase the staining intensity. The recommended time of incubation is 60 minutes at room temperature. |
Target
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Relevance
Function: Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. Tissue specificity: Placenta. Similarity: Belongs to the glycoprotein hormones subunit beta family. Developmental stage: Made by the first trimester placenta. -
Database links
- Entrez Gene: 114336 Human
- Omim: 118860 Human
- Omim: 608824 Human
- SwissProt: P01233 Human
- SwissProt: P0DN86 Human
- SwissProt: P0DN87 Human
- SwissProt: Q6NT52 Human
- Unigene: 172944 Human
see all -
Alternative names
- CG beta antibody
- CG-beta antibody
- CgB antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (ab9582)
Formalin-fixed, paraffin-embedded human seminoma tissue stained for hCG beta with ab9582 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (ab9582)Image from Spitalieri P et al, Cloning Stem Cells. 2009 Dec;11(4):535-56, Fig 1, DOI: 10.1089=clo.2009.0046.
ab9582 staining hCG beta in human fetal chorionic villus by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Samples were fixed in 4% paraformaldehyde and then incubated with ab9582 in PBT [3% bovine serum albumin (BSA) and 0.5% Triton X-100 in phosphate-buffered saline (PBS)] overnight at 4°C. After extensive washing with PBT, samples were incubated with a secondary antibody conjugated to Alexa-Fluor® 488 for 1 hour at room temperature in the dark. After nuclear staining with Hoechst slides were mounted with PBS/ glycerol.
Protocols
References (11)
ab9582 has been referenced in 11 publications.
- Drwal E et al. "Real life" polycyclic aromatic hydrocarbon (PAH) mixtures modulate hCG, hPL and hPLGF levels and disrupt the physiological ratio of MMP-2 to MMP-9 and VEGF expression in human placenta cell lines. Reprod Toxicol 95:1-10 (2020). PubMed: 32418891
- Lv B et al. Single-cell RNA sequencing reveals regulatory mechanism for trophoblast cell-fate divergence in human peri-implantation conceptuses. PLoS Biol 17:e3000187 (2019). PubMed: 31596842
- Li Z et al. Establishment of human trophoblast stem cells from human induced pluripotent stem cell-derived cystic cells under micromesh culture. Stem Cell Res Ther 10:245 (2019). PubMed: 31391109
- Sengodan SK et al. BRCA1 regulation on ß-hCG: a mechanism for tumorigenicity in BRCA1 defective breast cancer. Oncogenesis 6:e376 (2017). PubMed: 28869585
- Kojima J et al. Efficient production of trophoblast lineage cells from human induced pluripotent stem cells. Lab Invest 97:1188-1200 (2017). PubMed: 28287635