Anti-hCG beta antibody [5H4-E2] (ab9582)

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Thank you for contacting Abcam regarding ab9582.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

I regret to inform to that we have not tested it in our lab so I am afraid that we can't advise on the binding characteristics to PDMS (Polydimethylsiloxane) surface. As I have mentioned in my previous e-mail, ab9582 has not been tested in immunoprecipitation.

Apologies!

Thank you for your enquiry and your interest in our products.

I can confirm that ab9582 (Anti-hCG beta antibody [5H4-E2]), has not been tested for IP yet, though the isotype has been determined (IgG1).

Normally, we would suggest using Protein G for mouse IgG1 antibodies; whilst for moue IgG2a and mouse IgG2b can be used with both Protein A and G. Please could you advise your customer to take a look at this site for further information:

https://www.abcam.com/index.html?pageconfig=resource&rid=11385

I hope this helps and if I can assist further, please do not hesitate to contact me.

Thank you for your enquiries.

I can confirm that ab9582hCG beta antibody was raised against the beta subunit ofhCG only, and its reactivity was blocked by betasubunit of hCG, notthe alpha subunit. Therefore, we would not expect it to detect the alpha form.

Human chorionic gonadotropin is a heterodimeric protein, with an alpha and betasubunit:

The alpha subunit is 92 amino acids long and isidentical tothe alpha subunit ofluteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH).

The beta subunit is unique to hCG.It contains 145 amino acids, encoded by six highly-homologous genes that are arranged in tandem and inverted pairs on chromosome 19q13.3 - CGB (1, 2, 3, 5, 7, 8).

There is information on the beta subunit of hCG protein on the UniProt protein database page from the link on the datasheet:

http://www.uniprot.org/uniprot/P01233

There is information on the apha subunit on the following page:

http://www.uniprot.org/uniprot/P01215

I hope this will be helpful. I suggest the customer may like to considera further literature search in order to obtain further information.

If you have any further questions, please do not hesitate to contact me.

Thank you for your feedback regarding ab9582. You mention a band at a molecular weight similar to that of albumin, is this around 60-70KDa? I think the MW for albumin is about 66KDa. Could this band be explained by the fact the antibody recognizes the whole hCG beta and the C-terminal end part of hCG beta as well as the hCG dimer molecule? I believe the molecular weight of a monomer of hCG is about 38K, which is about half this weight. Please do not hesitate to contact me should you need further information,

Thank you for the information that you have provided. We have not had any other complaints concerning this antibody. Since you have not been able to obtain a signal with the new vial under the same conditions that you used with the previous lot, I would like to send you a replacement vial to try. It's a possibility that this latest vial went off during the shipping process. However, I do need either the Abcam order number or purchase order number that was used to order ab9582. Thank you, and I look forward to hearing from you.