Anti-hCG beta antibody (ab53087)

Submit a review Q&A (1)References (3)

Overview

  • Product name

    Anti-hCG beta antibody
    See all hCG beta primary antibodies

  • Description

    Rabbit polyclonal to hCG beta

  • Host species

    Rabbit

  • Specificity

    This antibody detects endogenous levels of total hCG beta protein.

  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide derived from human hCG beta.

  • Positive control

    • NIH/3T3 cell extract; Paraffin embedded human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab53087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    Function: Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. Tissue specificity: Placenta. Similarity: Belongs to the glycoprotein hormones subunit beta family. Developmental stage: Made by the first trimester placenta.

  • Database links

    see all

  • Alternative names

    • CG beta antibody
    • CG-beta antibody
    • CgB antibody
    • CGB3 antibody
    • CGB3_HUMAN antibody
    • CGB5 antibody
    • CGB7 antibody
    • CGB7_HUMAN antibody
    • CGB8 antibody
    • CGHB_HUMAN antibody
    • Choriogonadotropin subunit beta 3 antibody
    • Choriogonadotropin subunit beta 7 antibody
    • Choriogonadotropin subunit beta antibody
    • Chorionic gonadotropin beta polypeptide antibody
    • Chorionic gonadotropin chain beta antibody
    see all

Images

  • Western blot - Anti-hCG beta antibody (ab53087)
    Western blot - Anti-hCG beta antibody (ab53087)
    All lanes : Anti-hCG beta antibody (ab53087) at 1/300 dilution

    Lane 1 : NIH/3T3 cell extract
    Lane 2 : NIH/3T3 cell extract with peptide

    Predicted band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody (ab53087)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody (ab53087)
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using hCG ß antibody, ab53087, at 1/50 dilution.

References

This product has been referenced in:

  • Wu W  et al. ß-hCG promotes epithelial ovarian cancer metastasis through ERK/MMP2 signaling pathway. Cell Cycle 18:46-59 (2019). Read more (PubMed: 30582718) »
  • Liu N  et al. Human chorionic gonadotropin ß regulates epithelial-mesenchymal transition and metastasis in human ovarian cancer. Oncol Rep 38:1464-1472 (2017). Read more (PubMed: 28713970) »
See all 3 Publications for this product

Publishing research using ab53087? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

What hCG-related moleculesm (hCG consisted of Beta and alfa subunit, hCG-Beta, Nicked hCG, Nicked hCG-Beta, Nicked hCG missing Beta subunit C-terminal peptide, Hyperglycosylated hCG) can this product specificly bind with?

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Answer

After running a BLAST, the immunogenic sequence of ab53087 also shares a very high homology with the following molecules:



-Isoform 1 of Choriogonadotropin subunit beta variant 1, A6NKQ9, http://www.uniprot.org/uniprot/A6NKQ9#A6NKQ9



-Isoform 2 of Choriogonadotropin subunit beta variant 1, A6NKQ9, http://www.uniprot.org/uniprot/A6NKQ9#A6NKQ9-2



-Isoform 2 of Choriogonadotropin subunit beta, P01233, http://www.uniprot.org/uniprot/P01233#P01233-2



-Choriogonadotropin subunit beta variant 2, Q6NT52, http://www.uniprot.org/uniprot/Q6NT52.



The antibody has not yet been tested for reactivity with these variants but based on the high degree of identity between the immunogen and these other sequences there is a very good chance of cross reactivity.

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