Anti-hCG beta antibody (ab53087)

Submit a review Q&A (1)References (2)

Overview

  • Product name
    Anti-hCG beta antibody
    See all hCG beta primary antibodies
  • Description
    Rabbit polyclonal to hCG beta
  • Host species
    Rabbit
  • Specificity
    This antibody detects endogenous levels of total hCG beta protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human hCG beta.

  • Positive control
    • NIH/3T3 cell extract; Paraffin embedded human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab53087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

Target

Images

  • Western blot - Anti-hCG beta antibody (ab53087)
    Western blot - Anti-hCG beta antibody (ab53087)
    All lanes : Anti-hCG beta antibody (ab53087) at 1/300 dilution

    Lane 1 : NIH/3T3 cell extract
    Lane 2 : NIH/3T3 cell extract with peptide

    Predicted band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody (ab53087)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody (ab53087)
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using hCG ß antibody, ab53087, at 1/50 dilution.

References

This product has been referenced in:
  • Liu N  et al. Human chorionic gonadotropin ß regulates epithelial-mesenchymal transition and metastasis in human ovarian cancer. Oncol Rep 38:1464-1472 (2017). Read more (PubMed: 28713970) »
  • McDonald EA & Wolfe MW Adiponectin attenuation of endocrine function within human term trophoblast cells. Endocrinology 150:4358-65 (2009). Read more (PubMed: 19520781) »
See all 2 Publications for this product

Publishing research using ab53087? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

What hCG-related moleculesm (hCG consisted of Beta and alfa subunit, hCG-Beta, Nicked hCG, Nicked hCG-Beta, Nicked hCG missing Beta subunit C-terminal peptide, Hyperglycosylated hCG) can this product specificly bind with?

Read More
Answer

After running a BLAST, the immunogenic sequence of ab53087 also shares a very high homology with the following molecules:



-Isoform 1 of Choriogonadotropin subunit beta variant 1, A6NKQ9, http://www.uniprot.org/uniprot/A6NKQ9#A6NKQ9



-Isoform 2 of Choriogonadotropin subunit beta variant 1, A6NKQ9, http://www.uniprot.org/uniprot/A6NKQ9#A6NKQ9-2



-Isoform 2 of Choriogonadotropin subunit beta, P01233, http://www.uniprot.org/uniprot/P01233#P01233-2



-Choriogonadotropin subunit beta variant 2, Q6NT52, http://www.uniprot.org/uniprot/Q6NT52.



The antibody has not yet been tested for reactivity with these variants but based on the high degree of identity between the immunogen and these other sequences there is a very good chance of cross reactivity.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up