Key features and details
- Mouse monoclonal [8G9A2] to hCG receptor/LHR
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-hCG receptor/LHR antibody [8G9A2]
See all hCG receptor/LHR primary antibodies
DescriptionMouse monoclonal [8G9A2] to hCG receptor/LHR
Tested applicationsSuitable for: Flow Cyt, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Mouse hCG receptor/LHR aa 21-362.
Database link: P22888
- HepG2, Jurkat and SMMC-7721 cell lysates; Jurkat cells.
This product was changed from ascites to supernatant. Lot no’s high than GR273983-8 are from Tissue Culture Supernatant
This product was previously labelled as hCG receptor
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab204950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/200 - 1/400.|
|WB||1/500 - 1/2000. Predicted molecular weight: 78 kDa.|
|ICC/IF||1/200 - 1/1000.|
FunctionReceptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
Tissue specificityGonadal and thyroid cells.
Involvement in diseaseDefects in LHCGR are a cause of familial male precocious puberty (FMPP) [MIM:176410]; also known as testotoxicosis. In FMPP the receptor is constitutively activated.
Defects in LHCGR are the cause of luteinizing hormone resistance (LHR) [MIM:238320]; also known as Leydig cell hypoplasia in males. LHR is an autosomal recessive disorder characterized by unresponsiveness to luteinizing hormone, defective sexual development in males, and defective follicular development and ovulation, amenorrhea and infertility in females. Two forms of the disorder have been defined in males. Type 1 is a severe form characterized by complete 46,XY male pseudohermaphroditism, low testosterone and high luteinizing hormone levels, total lack of responsiveness to luteinizing and chorionic gonadotropin hormones, lack of breast development, and absent development of secondary male sex characteristics. Type 2, a milder form, displays a broader range of phenotypic expression ranging from micropenis to severe hypospadias.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
Contains 6 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain.
Cellular localizationCell membrane.
- Information by UniProt
- Gonadotropin receptor antibody
- Gpcr19-rs1 antibody
- GTHR-II antibody
All lanes : Anti-hCG receptor/LHR antibody [8G9A2] (ab204950) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : SMMC-7721 cell lysate
Predicted band size: 78 kDa
Flow cytometric analysis of Jurkat cells labeling hCG receptor/LHR with ab204950 at 1/200 dilution (green) compared with a negative control (red).
ab204950 has been referenced in 2 publications.
- Quinn MA et al. Estrogen Deficiency Promotes Hepatic Steatosis via a Glucocorticoid Receptor-Dependent Mechanism in Mice. Cell Rep 22:2690-2701 (2018). WB ; Mouse . PubMed: 29514097
- Zhao R et al. Human chorionic gonadotropin promotes cell proliferation through the activation of c-Met in gastric cancer cells. Oncol Lett 16:4271-4278 (2018). PubMed: 30197669