Overview

  • Product name

    Anti-hCG receptor/LHR antibody
    See all hCG receptor/LHR primary antibodies
  • Description

    Rabbit polyclonal to hCG receptor/LHR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human hCG receptor/LHR (N terminal).

  • General notes

     This product was previously labelled as hCG receptor

     

Properties

Applications

Our Abpromise guarantee covers the use of ab125214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 79 kDa.

The detection limit for ab125214 is approximately 0.1ng/lane under non-reducing and reducing conditions.

IHC-P Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
  • Tissue specificity

    Gonadal and thyroid cells.
  • Involvement in disease

    Defects in LHCGR are a cause of familial male precocious puberty (FMPP) [MIM:176410]; also known as testotoxicosis. In FMPP the receptor is constitutively activated.
    Defects in LHCGR are the cause of luteinizing hormone resistance (LHR) [MIM:238320]; also known as Leydig cell hypoplasia in males. LHR is an autosomal recessive disorder characterized by unresponsiveness to luteinizing hormone, defective sexual development in males, and defective follicular development and ovulation, amenorrhea and infertility in females. Two forms of the disorder have been defined in males. Type 1 is a severe form characterized by complete 46,XY male pseudohermaphroditism, low testosterone and high luteinizing hormone levels, total lack of responsiveness to luteinizing and chorionic gonadotropin hormones, lack of breast development, and absent development of secondary male sex characteristics. Type 2, a milder form, displays a broader range of phenotypic expression ranging from micropenis to severe hypospadias.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
    Contains 6 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Gonadotropin receptor antibody
    • Gpcr19-rs1 antibody
    • GTHR-II antibody
    • HHG antibody
    • LCGR antibody
    • LGR2 antibody
    • LH-R antibody
    • LH/CG R antibody
    • LH/CG-R antibody
    • LH/CGR antibody
    • LHCGR antibody
    • LHR antibody
    • LHRHR antibody
    • LSH R antibody
    • LSH-R antibody
    • LSHR_HUMAN antibody
    • Luteinizing hormone receptor antibody
    • Luteinizing hormone/choriogonadotropin receptor antibody
    • Lutropin choriogonadotropic hormone receptor antibody
    • Lutropin choriogonadotropic receptor antibody
    • Lutropin-choriogonadotropic hormone receptor antibody
    • ULG5 antibody
    see all

Images

  •  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining hCG receptor/LHR in Rat Ovary Tissue.
     
  • Anti-hCG receptor/LHR antibody (ab125214) at 0.5 µg/ml + MCF-7 Whole Cell Lysate at 40 µg

    Predicted band size: 79 kDa

References

This product has been referenced in:

  • Zhang H  et al. Seasonal expressions of follicle-stimulating hormone receptor and luteinizing hormone receptor in the scented gland of the male muskrat (Ondatra zibethicus). Am J Physiol Regul Integr Comp Physiol 312:R569-R574 (2017). Read more (PubMed: 28228419) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

This product is tested for rat and Immunohistochemistry in Paraffin sections. We do generally recommend using a heat mediated antigen retrieval step, such as with citrate buffer. This antibody is against Hcg receptor, which is expressed on the cell membrane.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

We do not generate this antibody in-house and the supplying laboratory considers the immunogen sequence to be proprietary. All we know is that the immunogen is A synthetic peptide corresponding to a sequence at the N-terminal of human LHCGR, different from the relative rat sequence by two amino acids, mouse sequence by three amino acids.

If you want to know if this antibody will work in a different species, let me know and I will ask the lab to BLAST the immunogen against that species to estimate the homology.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you very much for your interest in ab125214.

Unfortunately we do not have any IHC images that I can send you at the moment for ab125214. To our knowledge, ab125214 has not been tested in ICC. Therefore, I can offer a discount off a future purchase if you buy ab125214 now, test it inICC and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab125214 in ICC. I will then send a discount code. This code must be issued before purchasing ab125214 so please wait for my reply before ordering.

2. Purchase ab125214 either by phone, fax, or online (www.abcam.com).

3. Test it in ICC.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab125214 turns out to be unsuitable for ICC, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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