Overview

  • Product name

    Anti-Hck (phospho Y410) antibody
    See all Hck primary antibodies
  • Description

    Rabbit polyclonal to Hck (phospho Y410)
  • Host species

    Rabbit
  • Specificity

    Detects target only when phosphorylated at tyrosine 410.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide derived from human Hck around the phosphorylation site of tyrosine 410 (N-E-YP-T-A)

  • Positive control

    • HeLa cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.
ELISA 1/20000.

Target

  • Function

    May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
  • Tissue specificity

    Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Domain

    The SH3 domain mediates binding to HIV-1 Nef.
  • Cellular localization

    Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
  • Information by UniProt
  • Database links

  • Form

    This protein is known to be similar in amino acid sequence to LCK (P06239), FYN (P06241), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names

    • Bmk antibody
    • Hck 1 antibody
    • Hck antibody
    • HCK_HUMAN antibody
    • Hemopoietic cell kinase antibody
    • JTK9 antibody
    • p59-HCK/p60-HCK antibody
    • p59Hck antibody
    • p59HCK/p60HCK antibody
    • p61Hck antibody
    • Tyrosine protein kinase HCK antibody
    • Tyrosine-protein kinase HCK antibody
    see all

Images

  • ab61055 staining Hck (phospho Y410) in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% BSA for 1 hour; antigen retrieval was by heat mediation in trypsin. Samples were incubated with primary antibody (1/100) for 24 hours. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • 1/500-1/1000 ab61055 staining phosphorilated Hck on HeLa cells in the absence (left) or presence (right) of the immunizing phosphopeptide.

References

This product has been referenced in:

  • Poh AR  et al. Inhibition of Hematopoietic Cell Kinase Activity Suppresses Myeloid Cell-Mediated Colon Cancer Progression. Cancer Cell 31:563-575.e5 (2017). Read more (PubMed: 28399411) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Trypsin
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
10% Formalin

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Verified customer

Submitted Oct 30 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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