Anti-HCLS1 antibody (ab227247)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-HCLS1 antibody
See all HCLS1 primary antibodies -
Description
Rabbit polyclonal to HCLS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human HCLS1 (internal sequence). The exact sequence is proprietary.
Database link: P14317 -
Positive control
- WB: Raji whole cell lysate. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab227247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 54 kDa. | |
| IP | 1/100 - 1/500. |
Target
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Function
Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. -
Tissue specificity
Expressed only in tissues and cells of hematopoietic origin. -
Sequence similarities
Contains 4 cortactin repeats.
Contains 1 SH3 domain. -
Developmental stage
Expressed in early stage of myeloid and erythroid differentiation. -
Post-translational
modificationsPhosphorylated by LYN; rapidly after cross-linking of surface IgM on B-cells. -
Cellular localization
Membrane. Cytoplasm. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 508445 Cow
- Entrez Gene: 3059 Human
- Entrez Gene: 15163 Mouse
- Entrez Gene: 288077 Rat
- Entrez Gene: 714044 Rhesus monkey
- Omim: 601306 Human
- SwissProt: P14317 Human
- SwissProt: P49710 Mouse
see all -
Alternative names
- Cortactin like antibody
- CTTNL antibody
- HCLS 1 antibody
see all
Images
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Western blot - Anti-HCLS1 antibody (ab227247)Anti-HCLS1 antibody (ab227247) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 54 kDa10% SDS-PAGE gel.
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Immunoprecipitation - Anti-HCLS1 antibody (ab227247)HCLS1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2.5 µg ab227247. Western blot was performed from the immunoprecipitate using ab227247 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HEK-293T whole cell lysate 30 µg.
Lane 1: Control IP in HEK-293T whole cell lysate with 2.5 μg of pre-immune rabbit IgG.
Lane 2: ab227247 IP in HEK-293T whole cell lysate.
10% SDS-PAGE gel.
References
ab227247 has not yet been referenced specifically in any publications.
