Anti-HCLS1 antibody (ab227247)
Key features and details
- Rabbit polyclonal to HCLS1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HCLS1 antibody
See all HCLS1 primary antibodies -
Description
Rabbit polyclonal to HCLS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human HCLS1 (internal sequence). The exact sequence is proprietary.
Database link: P14317 -
Positive control
- WB: Raji whole cell lysate. IP: HEK-293T whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab227247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 54 kDa. | |
| IP | 1/100 - 1/500. |
Target
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Function
Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. -
Tissue specificity
Expressed only in tissues and cells of hematopoietic origin. -
Sequence similarities
Contains 4 cortactin repeats.
Contains 1 SH3 domain. -
Developmental stage
Expressed in early stage of myeloid and erythroid differentiation. -
Post-translational
modificationsPhosphorylated by LYN; rapidly after cross-linking of surface IgM on B-cells. -
Cellular localization
Membrane. Cytoplasm. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 508445 Cow
- Entrez Gene: 3059 Human
- Entrez Gene: 15163 Mouse
- Entrez Gene: 288077 Rat
- Entrez Gene: 714044 Rhesus monkey
- Omim: 601306 Human
- SwissProt: P14317 Human
- SwissProt: P49710 Mouse
see all -
Alternative names
- Cortactin like antibody
- CTTNL antibody
- HCLS 1 antibody
see all
Images
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Western blot - Anti-HCLS1 antibody (ab227247)Anti-HCLS1 antibody (ab227247) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 54 kDa10% SDS-PAGE gel.
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Immunoprecipitation - Anti-HCLS1 antibody (ab227247)HCLS1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with 2.5 µg ab227247. Western blot was performed from the immunoprecipitate using ab227247 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HEK-293T whole cell lysate 30 µg.
Lane 1: Control IP in HEK-293T whole cell lysate with 2.5 μg of pre-immune rabbit IgG.
Lane 2: ab227247 IP in HEK-293T whole cell lysate.
10% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227247 has not yet been referenced specifically in any publications.
