Product nameHCLS1 overexpression 293T lysate (whole cell)
ab94153 is a 293T cell transfected lysate in which Human HCLS1 has been transiently over-expressed using a pCMV-HCLS1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundDevelopmental stage: Expressed in early stage of myeloid and erythroid differentiation. Function: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. PTM: Phosphorylated by LYN; rapidly after cross-linking of surface IgM on B-cells. Similarity: Contains 4 cortactin repeats. Contains 1 SH3 domain. Tissue specificity: Expressed only in tissues and cells of hematopoietic origin.
Our Abpromise guarantee covers the use of ab94153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94153 at 15µg/lane on an SDS PAGE gel.
All lanes : Anti-HCLS1 antibody (ab55090) at 1/500 dilution
Lane 1 :
HCLS1 overexpression 293T lysate (whole cell) (ab94153)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94153 has not yet been referenced specifically in any publications.