Anti-HCN1 antibody (ab176304)
Key features and details
- Rabbit polyclonal to HCN1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HCN1 antibody
See all HCN1 primary antibodies -
Description
Rabbit polyclonal to HCN1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant full length protein corresponding to HCN1 aa 1-890.
Sequence:MEGGGKPNSS SNSRDDGNSV FPAKASATGA GPAAAEKRLG TPPGGGGAGA KEHGNSVCFK VDGGGGGGGG GGGGEEPAGG FEDAEGPRRQ YGFMQRQFTS MLQPGVNKFS LRMFGSQKAV EKEQERVKTA GFWIIHPYSD FRFYWDLIML IMMVGNLVII PVGITFFTEQ TTTPWIIFNV ASDTVFLLDL IMNFRTGTVN EDSSEIILDP KVIKMNYLKS WFVVDFISSI PVDYIFLIVE KGMDSEVYKT ARALRIVRFT KILSLLRLLR LSRLIRYIHQ WEEIFHMTYD LASAVVRIFN LIGMMLLLCH WDGCLQFLVP LLQDFPPDCW VSLNEMVNDS WGKQYSYALF KAMSHMLCIG YGAQAPVSMS DLWITMLSMI VGATCYAMFV GHATALIQSL DSSRRQYQEK YKQVEQYMSF HKLPADMRQK IHDYYEHRYQ GKIFDEENIL NELNDPLREE IVNFNCRKLV ATMPLFANAD PNFVTAMLSK LRFEVFQPGD YIIREGAVGK KMYFIQHGVA GVITKSSKEM KLTDGSYFGE ICLLTKGRRT ASVRADTYCR LYSLSVDNFN EVLEEYPMMR RAFETVAIDR LDRIGKKNSI LLQKFQKDLN TGVFNNQENE ILKQIVKHDR EMVQAIAPIN YPQMTTLNST SSTTTPTSRM RTQSPPVYTA TSLSHSNLHS PSPSTQTPQP SAILSPCSYT TAVCSPPVQS PLAARTFHYA SPTASQLSLM QQQPQQQVQQ SQPPQTQPQQ PSPQPQTPGS STPKNEVHKS TQALHNTNLT REVRPLSASQ PSLPHEVSTL ISRPHPTVGE SLASIPQPVT AVPGTGLQAG GRSTVPQRVT LFRQMSSGAI PPNRGVPPAP PPPAAALPRE SSSVLNTDPD AEKPRFASNL
Database link: O60741 -
Positive control
- WB: 293T, MCF7, OVCAR3 and SH-SY5Y. Mouse brain, eye, ovary and lung tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 99 kDa.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 99 kDa. |
Target
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Function
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli. -
Tissue specificity
Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas. -
Sequence similarities
Belongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 348980 Human
- Entrez Gene: 15165 Mouse
- Entrez Gene: 84390 Rat
- Omim: 602780 Human
- SwissProt: O60741 Human
- SwissProt: O88704 Mouse
- SwissProt: Q9JKB0 Rat
- Unigene: 650434 Human
see all -
Alternative names
- BCNG-1 antibody
- BCNG1 antibody
- Brain cyclic nucleotide gated channel 1 antibody
see all
Images
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All lanes : Anti-HCN1 antibody (ab176304) at 1/400 dilution
Lane 1 : 293T cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : OVCAR3 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse eye tissue lysate
Lane 7 : Mouse ovary tissue lysate
Lane 8 : Mouse lung tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 99 kDa
Exposure time: 90 secondsBlocking buffer: 3% nonfat dry milk in TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab176304 has been referenced in 2 publications.
- Rátkai A et al. Homeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and T-type calcium channels in neuronal cultures. Sci Rep 11:3236 (2021). PubMed: 33547341
- Datson NA et al. The expanded CAG repeat in the huntingtin gene as target for therapeutic RNA modulation throughout the HD mouse brain. PLoS One 12:e0171127 (2017). PubMed: 28182673