Overview

  • Product name

    Anti-HCN1 antibody - C-terminal
    See all HCN1 primary antibodies
  • Description

    Rabbit polyclonal to HCN1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human HCN1 (C terminal). The exact sequence is proprietary.
    Database link: O60741

  • Positive control

    • WB: Mouse and rat brain tissue extracts; SK-N-AS whole cell extract. IHC-P: Mouse retina and brain tissues; rat brain tissue. ICC/IF: Rat DIV9 E18 primary cortical neurons. IHC-Fr: Frozen mouse cerebellum and hippocampus tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab229340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 99 kDa.

Target

  • Function

    Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli.
  • Tissue specificity

    Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.
  • Sequence similarities

    Belongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.
  • Domain

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BCNG-1 antibody
    • BCNG1 antibody
    • Brain cyclic nucleotide gated channel 1 antibody
    • Brain cyclic nucleotide-gated channel 1 antibody
    • HAC 2 antibody
    • HAC2 antibody
    • HCN1 antibody
    • HCN1_HUMAN antibody
    • Hyperpolarization activated cyclic nucleotide gated potassium channel 1 antibody
    • Potassium channel, voltage-gated, brain, 1 antibody
    • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 antibody
    see all

Images

  • Anti-HCN1 antibody - C-terminal (ab229340) at 1/1000 dilution + SK-N-AS (human neuroblastoma cell line) whole cell extract at 30 µg

    Predicted band size: 99 kDa



    7.5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons cells stained for HCN1 (green) using ab229340 at 1/500 dilution in ICC/IF.

    Beta Tubulin 3/ Tuj1 (red).

    Nuclear counterstain: Fluoroshield with DAPI (blue).

  • Paraffin-embedded rat brain tissue stained for HCN1 using ab229340 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse brain tissue stained for HCN1 using ab229340 at 1/500 dilution in immunohistochemical analysis.

  • Frozen-sectioned adult mouse hippocampus tissue stained for HCN1 (green) using ab229340 at 1/250 dilution in immunohistochemical analysis.

    Nuclear counterstain: Fluoroshield with DAPI (blue)

  • All lanes : Anti-HCN1 antibody - C-terminal (ab229340) at 1/1000 dilution

    Lane 1 : Mouse brain tissue extract
    Lane 2 : Rat brain tissue extract

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 99 kDa



    7.5% SDS-PAGE gel.

  • Frozen-sectioned adult mouse cerebellum tissue stained for HCN1 (green) using ab229340 at 1/250 dilution in immunohistochemical analysis.

    beta Tubulin 3/ TUJ1 (red).

    Nuclear counterstain: Fluoroshield with DAPI (blue).

  • Paraffin-embedded mouse retina tissue stained for HCN1 using ab229340 at 1/800 dilution in immunohistochemical abalysis.

References

ab229340 has not yet been referenced specifically in any publications.

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