Anti-HCN2 antibody (ab19346)

Submit a review Q&A (1)References (5)
Western blot - Anti-HCN2 antibody (ab19346)

    Key features and details

    • Rabbit polyclonal to HCN2
    • Suitable for: WB
    • Reacts with: Rat, Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-HCN2 antibody
      See all HCN2 primary antibodies

    • Description

      Rabbit polyclonal to HCN2

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide corresponding to Human HCN2 aa 874-889.
      Sequence:

      G-LDPQDSARSRLSSNL


      Database link: Q9UL51
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • rat brain lysate

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab19346 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Application Abreviews Notes
    WB
    1/100. Detects a band of approximately 100,115 kDa.
    Notes
    WB
    1/100. Detects a band of approximately 100,115 kDa.

    Target

    • Function

      Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages.

    • Tissue specificity

      Highly expressed throughout the brain. Detected at low levels in heart.

    • Sequence similarities

      Belongs to the potassium channel HCN family.
      Contains 1 cyclic nucleotide-binding domain.

    • Domain

      The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • BCNG-2 antibody
      • BCNG2 antibody
      • Brain cyclic nucleotide gated channel 2 antibody
      • Brain cyclic nucleotide-gated channel 2 antibody
      • HAC1 antibody
      • Hcn2 antibody
      • HCN2_HUMAN antibody
      • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 antibody
      see all

    Images

    • Western blot - Anti-HCN2 antibody (ab19346)
      Western blot - Anti-HCN2 antibody (ab19346)

      ab19346 has been successfully used in Western blot procedures. By Western blot, this antibody detects bands at ~100 kDa and ~115 kDa representing the non-glycosylated and glycosylated form of HCN2 in rat brain extract.

    References (5)

    Publishing research using ab19346? Please let us know so that we can cite the reference in this datasheet.

    ab19346 has been referenced in 5 publications.

    • Zhang W  et al. Implantation of engineered conduction tissue in the rat heart. Mol Med Rep 19:2687-2697 (2019). PubMed: 30720107
    • Yang L  et al. HCN channel antagonist ZD7288 ameliorates neuropathic pain and associated depression. Brain Res 1717:204-213 (2019). PubMed: 30940440
    • Kretschmannova K  et al. The expression and role of hyperpolarization-activated and cyclic nucleotide-gated channels in endocrine anterior pituitary cells. Mol Endocrinol 26:153-64 (2012). WB ; Rat . PubMed: 22135067
    • Adams BE  et al. Excitotoxic-mediated transcriptional decreases in HCN2 channel function increase network excitability in CA1. Exp Neurol 219:249-57 (2009). WB . PubMed: 19500574
    • Fyk-Kolodziej B & Pourcho RG Differential distribution of hyperpolarization-activated and cyclic nucleotide-gated channels in cone bipolar cells of the rat retina. J Comp Neurol 501:891-903 (2007). PubMed: 17311321

    Customer reviews and Q&As

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    Question

    Can you tell me where I can purchase the immunising peptide for this antibody and what their Western blot protocol is including the preparation of lysates?

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    Answer

    Thank you for your enquiry. Unfortunately we do not sell the immunising peptide for this antibody. The Western blot protocol used to test this antibody can be found under our Protocol tab. Regarding lysate prep, you should be able to use a standard lysis buffer (75 mM Tris-HCl, pH 7.2, 200 mM NaCl, 1% Tween 100, 100 mM EDTA, 1 mM DTT with protease inhibitors). Please note, HCN2 has an N-linked glycosylation in the brain. This antibody detects a band at ~115 kDa (due to glycosylation). However this band can be reduced to ~100 kDa by the addition of PNGase F. You may find the deglycosylation procedure in this specific reference. http://www.jbc.org/cgi/reprint/M306958200v1 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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