Anti-HCN2 antibody (ab19346)
Key features and details
- Rabbit polyclonal to HCN2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-HCN2 antibody
See all HCN2 primary antibodies -
Description
Rabbit polyclonal to HCN2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide corresponding to Human HCN2 aa 874-889.
Sequence:G-LDPQDSARSRLSSNL
Database link: Q9UL51 -
Positive control
- WB: rat brain lysate ICC/IF: Neuro-2a (Mouse neuroblastoma cell line)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/100. Detects a band of approximately 100,115 kDa.
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| ICC/IF |
Use a concentration of 2 µg/ml.
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| Notes |
|---|
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WB
1/100. Detects a band of approximately 100,115 kDa. |
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ICC/IF
Use a concentration of 2 µg/ml. |
Target
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Function
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. -
Tissue specificity
Highly expressed throughout the brain. Detected at low levels in heart. -
Sequence similarities
Belongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 610 Human
- Entrez Gene: 15166 Mouse
- Entrez Gene: 114244 Rat
- Omim: 602781 Human
- SwissProt: Q9UL51 Human
- SwissProt: O88703 Mouse
- SwissProt: Q9JKA9 Rat
- Unigene: 124161 Human
see all -
Alternative names
- BCNG-2 antibody
- BCNG2 antibody
- Brain cyclic nucleotide gated channel 2 antibody
see all
Images
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Western blot - Anti-HCN2 antibody (ab19346)
ab19346 has been successfully used in Western blot procedures. By Western blot, this antibody detects bands at ~100 kDa and ~115 kDa representing the non-glycosylated and glycosylated form of HCN2 in rat brain extract.
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Immunocytochemistry/ Immunofluorescence - Anti-HCN2 antibody (ab19346)Immunofluorescent analysis of Neuro-2a (Mouse neuroblastoma cell line) cells on 70% confluent log phase labeling HCN2. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labelled with ab19346 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Datasheets and documents
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Datasheet download
References (7)
ab19346 has been referenced in 7 publications.
- Lu Z et al. Regulation of HCN2 Current by PI3K/Akt Signaling. Front Physiol 11:587040 (2020). PubMed: 33240105
- Zhao H et al. Overexpression of the medium-conductance calcium-activated potassium channel (SK4) and the HCN2 channel to generate a biological pacemaker. Mol Med Rep 20:3406-3414 (2019). PubMed: 31432175
- Zhang W et al. Implantation of engineered conduction tissue in the rat heart. Mol Med Rep 19:2687-2697 (2019). PubMed: 30720107
- Yang L et al. HCN channel antagonist ZD7288 ameliorates neuropathic pain and associated depression. Brain Res 1717:204-213 (2019). PubMed: 30940440
- Kretschmannova K et al. The expression and role of hyperpolarization-activated and cyclic nucleotide-gated channels in endocrine anterior pituitary cells. Mol Endocrinol 26:153-64 (2012). WB ; Rat . PubMed: 22135067
- Adams BE et al. Excitotoxic-mediated transcriptional decreases in HCN2 channel function increase network excitability in CA1. Exp Neurol 219:249-57 (2009). WB . PubMed: 19500574
- Fyk-Kolodziej B & Pourcho RG Differential distribution of hyperpolarization-activated and cyclic nucleotide-gated channels in cone bipolar cells of the rat retina. J Comp Neurol 501:891-903 (2007). PubMed: 17311321
