Anti-HCN2 antibody (ab19346)

Submit a review Q&A (1)References (3)

Overview

  • Product name
    Anti-HCN2 antibody
    See all HCN2 primary antibodies
  • Description
    Rabbit polyclonal to HCN2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human HCN2 aa 874-889.
    Sequence:

    G-LDPQDSARSRLSSNL


    Database link: Q9UL51

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • rat brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab19346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 100,115 kDa.

Target

  • Function
    Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages.
  • Tissue specificity
    Highly expressed throughout the brain. Detected at low levels in heart.
  • Sequence similarities
    Belongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.
  • Domain
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • BCNG-2 antibody
    • BCNG2 antibody
    • Brain cyclic nucleotide gated channel 2 antibody
    • Brain cyclic nucleotide-gated channel 2 antibody
    • HAC1 antibody
    • Hcn2 antibody
    • HCN2_HUMAN antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 antibody
    see all

Images

  • Western blot - Anti-HCN2 antibody (ab19346)
    Western blot - Anti-HCN2 antibody (ab19346)

    ab19346 has been successfully used in Western blot procedures. By Western blot, this antibody detects bands at ~100 kDa and ~115 kDa representing the non-glycosylated and glycosylated form of HCN2 in rat brain extract.

References

This product has been referenced in:
  • Kretschmannova K  et al. The expression and role of hyperpolarization-activated and cyclic nucleotide-gated channels in endocrine anterior pituitary cells. Mol Endocrinol 26:153-64 (2012). WB ; Rat . Read more (PubMed: 22135067) »
  • Adams BE  et al. Excitotoxic-mediated transcriptional decreases in HCN2 channel function increase network excitability in CA1. Exp Neurol 219:249-57 (2009). WB . Read more (PubMed: 19500574) »
See all 3 Publications for this product

Publishing research using ab19346? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Can you tell me where I can purchase the immunising peptide for this antibody and what their Western blot protocol is including the preparation of lysates?

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Answer

Thank you for your enquiry. Unfortunately we do not sell the immunising peptide for this antibody. The Western blot protocol used to test this antibody can be found under our Protocol tab. Regarding lysate prep, you should be able to use a standard lysis buffer (75 mM Tris-HCl, pH 7.2, 200 mM NaCl, 1% Tween 100, 100 mM EDTA, 1 mM DTT with protease inhibitors). Please note, HCN2 has an N-linked glycosylation in the brain. This antibody detects a band at ~115 kDa (due to glycosylation). However this band can be reduced to ~100 kDa by the addition of PNGase F. You may find the deglycosylation procedure in this specific reference. http://www.jbc.org/cgi/reprint/M306958200v1 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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