Anti-HCN2 antibody [S71] (ab84817)
![Immunocytochemistry - Anti-HCN2 antibody [S71] (ab84817)](https://www.abcam.com/ps/products/84/ab84817/Images/ab84817-453255-anti-hcn2-antibody-s71-immunocytochemistry-shsy5y-human.jpg "Immunocytochemistry - Anti-HCN2 antibody [S71] (ab84817)")
Key features and details
- Mouse monoclonal [S71] to HCN2
- Suitable for: IHC-Fr, IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-HCN2 antibody [S71]
See all HCN2 primary antibodies -
Description
Mouse monoclonal [S71] to HCN2 -
Host species
Mouse -
Specificity
Does not cross react with HCN1. -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Fusion protein corresponding to Rat HCN2 aa 750 to the C-terminus (C terminal).
Database link: Q9JKA9 -
Positive control
- IHC-Fr: Mouse brain tissue; IHC-P: Human hippocampus tissue; WB: Rat brain membrane and mouse brain tissue lysates; ICC: Differentiated SH-SH5Y cells.
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General notes
The clone number S71 has previously been called S71-37 and N71/37. These clone numbers all refer to the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
S71 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/1000.
|
|
| IHC-P |
1/100.
|
|
| ICC |
1/100.
|
|
| WB | (3) |
1/1000. Predicted molecular weight: 95 kDa.
|
| Notes |
|---|
|
IHC-Fr
1/1000. |
|
IHC-P
1/100. |
|
ICC
1/100. |
|
WB
1/1000. Predicted molecular weight: 95 kDa. |
Target
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Function
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. -
Tissue specificity
Highly expressed throughout the brain. Detected at low levels in heart. -
Sequence similarities
Belongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 610 Human
- Entrez Gene: 15166 Mouse
- Entrez Gene: 114244 Rat
- Omim: 602781 Human
- SwissProt: Q9UL51 Human
- SwissProt: O88703 Mouse
- SwissProt: Q9JKA9 Rat
- Unigene: 124161 Human
see all -
Alternative names
- BCNG-2 antibody
- BCNG2 antibody
- Brain cyclic nucleotide gated channel 2 antibody
see all
Images
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Immunocytochemistry - Anti-HCN2 antibody [S71] (ab84817)
Immunocytochemistry analysis of differentiated SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labelling HCN2 with ab84817 at 1/100 dilution followed by secondary antibody (Alexa Fluor® 488) (green). Cells were counterstained with phalloidin (Alexa Fluor® 647) (red), and with Anti-beta III Tubulin antibody (Alexa Fluor® 555) (magenta). Nuclear DNA was labelled with Hoechst (blue).
(A) Phalloidin
(B) Anti-beta III Tubulin antibody
(C) HCN2 Antibody (ab84817) and secondary antibody
(D) Hoechst
(E) Composite
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [S71] (ab84817)](https://www.abcam.com/ps/products/84/ab84817/Images/ab84817-453248-anti-hcn2-antibody-s71-immunohistochemistry-hippocampus-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [S71] (ab84817)Immunohistochemistry (Bouin-fixed paraffin-embedded sections) analysis of human hippocampus tissue labelling HCN2 with ab84817 at 1/100 dilution (1 hour at room temperature). FITC Goat Anti-Mouse (green) was used as the secondary antibody at 1/50 dilution (1 hour at room temperature).
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![Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (ab84817)](https://www.abcam.com/ps/products/84/ab84817/Images/ab84817-199537-anti-hcn2-antibody-n71-37-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (ab84817)Immunohistochemistry (Frozen sections) analysis of mouse brain tissue labelling HCN2 with ab84817 at 1/1000 dilution (1 hour at room temperature). Tissue fixed with 10% Formalin Solution for 12-24 hours at room temperature. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstained with Mayer hematoxylin (purple/blue) nuclear stain at 250-500 µL for 5 minutes at room temperature.
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![Western blot - Anti-HCN2 antibody [S71] (ab84817)](https://www.abcam.com/ps/products/84/ab84817/Images/ab84817-369636-anti-hcn2-antibody-n71-37-westernblot-mouse-brain.jpg)
Western blot - Anti-HCN2 antibody [S71] (ab84817)Anti-HCN2 antibody [S71] (ab84817) at 1/1000 dilution + Mouse brain tissue lysate at 15 µg
Secondary
Goat anti-mouse IgG, HRP at 1/4000 dilution
Predicted band size: 95 kDa
Observed band size: 95 kDaChemiluminescent for HRP (Moss) for 5 min in RT.
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![Western blot - Anti-HCN2 antibody [S71] (ab84817)](https://www.abcam.com/ps/products/84/ab84817/Images/ab84817-199538-anti-hcn2-antibody-n71-37-western-blot.jpg)
Western blot - Anti-HCN2 antibody [S71] (ab84817)Anti-HCN2 antibody [S71] (ab84817) at 1/1000 dilution + Rat brain membrane lysates at 15 µg
Secondary
HRP-Sheep Anti-Mouse IgG antibody
Predicted band size: 95 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (9)
ab84817 has been referenced in 9 publications.
- Zhong W & Darmani NA The HCN Channel Blocker ZD7288 Induces Emesis in the Least Shrew (Cryptotis parva). Front Pharmacol 12:647021 (2021). PubMed: 33995059
- Zhang X et al. Shank3 contributes to neuropathic pain by facilitating the SNI-dependent increase of HCN2 and the expression of PSD95. Neurosci Res N/A:N/A (2020). PubMed: 32454040
- Zhao H et al. Overexpression of the medium-conductance calcium-activated potassium channel (SK4) and the HCN2 channel to generate a biological pacemaker. Mol Med Rep 20:3406-3414 (2019). PubMed: 31432175
- Mader F et al. Hyperpolarization-Activated Cyclic Nucleotide-Gated Non-selective (HCN) Ion Channels Regulate Human and Murine Urinary Bladder Contractility. Front Physiol 9:753 (2018). PubMed: 29971015
- Ding W et al. Increased HCN Channel Activity in the Gasserian Ganglion Contributes to Trigeminal Neuropathic Pain. J Pain 19:626-634 (2018). PubMed: 29366880
- Guo T et al. Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites. Oncol Lett 13:1601-1608 (2017). IHC-Fr ; Mouse . PubMed: 28454297
- Pitcairn E et al. Coordinating heart morphogenesis: A novel role for hyperpolarization-activated cyclic nucleotide-gated (HCN) channels during cardiogenesis inXenopus laevis. Commun Integr Biol 10:e1309488 (2017). PubMed: 28702127
- Ding W et al. Inhibition of HCN channel activity in the thalamus attenuates chronic pain in rats. Neurosci Lett 631:97-103 (2016). PubMed: 27542339
- Feng QF et al. The effects of acupuncture on bladder interstitial cells of cajal excitability in rats with overactive bladder. Evid Based Complement Alternat Med 2013:261217 (2013). IHC . PubMed: 24194780
