Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab109807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 86 kDa).|
FunctionPutative hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions.
Sequence similaritiesBelongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain.
DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
- Information by UniProt
- Hcn3 antibody
- HCN3_HUMAN antibody
- hyperpolarization activated cyclic nucleotide-gated potassium channel 3 antibody
Anti-HCN3 antibody (ab109807) at 1 µg/ml + Rat kidney lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
This product has been referenced in:
- Kretschmannova K et al. The expression and role of hyperpolarization-activated and cyclic nucleotide-gated channels in endocrine anterior pituitary cells. Mol Endocrinol 26:153-64 (2012). WB ; Rat . Read more (PubMed: 22135067) »