Recombinant Anti-HCN3 antibody [EPR13494(2)] (ab192025)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13494(2)] to HCN3
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-HCN3 antibody [EPR13494(2)]
See all HCN3 primary antibodies -
Description
Rabbit monoclonal [EPR13494(2)] to HCN3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human cerebellum, fetal brain, forebrain and hypothalamus lysates; Mouse and Rat brain tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13494(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
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IP |
1/30.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa). |
IP
1/30. |
Target
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Function
Putative hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. -
Sequence similarities
Belongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 57657 Human
- Entrez Gene: 15168 Mouse
- Entrez Gene: 114245 Rat
- Omim: 609973 Human
- SwissProt: Q9P1Z3 Human
- SwissProt: O88705 Mouse
- SwissProt: Q9JKA8 Rat
- Unigene: 706960 Human
see all -
Alternative names
- Hcn3 antibody
- HCN3_HUMAN antibody
- hyperpolarization activated cyclic nucleotide-gated potassium channel 3 antibody
see all
Images
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All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 0.511 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat Brain Tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM /TBST
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All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 1/10000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 86 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 1/1000 dilution
Lane 1 : Human forebrain lysate
Lane 2 : Human hypothalamus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 86 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
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Western blot analysis of HCN3 in Human fetal brain lysate immunoprecipitated using ab192025 at 1/30 dilution.
Secondary antibody: Goat anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution.
Blocking/dilution buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab192025 has been referenced in 1 publication.
- He F et al. The role of HCN channels in peristaltic dysfunction in human ureteral tuberculosis. Int Urol Nephrol 50:639-645 (2018). WB . PubMed: 29460132