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  1. Link

    hcn3-antibody-epr134942-ab192025.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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RecombinantRabMAb

Recombinant Anti-HCN3 antibody [EPR13494(2)] (ab192025)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
  • Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
  • Anti-HCN3 antibody [EPR13494(2)] (ab192025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13494(2)] to HCN3
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-HCN3 antibody [EPR13494(2)]
    See all HCN3 primary antibodies
  • Description

    Rabbit monoclonal [EPR13494(2)] to HCN3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human cerebellum, fetal brain, forebrain and hypothalamus lysates; Mouse and Rat brain tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13494(2)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • More Ion Channels
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Cardiovascular
    • Heart
    • Contractility
    • Inotropics
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (ab251107)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab192025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
IP
1/30.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
IP
1/30.

Target

  • Function

    Putative hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions.
  • Sequence similarities

    Belongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.
  • Domain

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q9P1Z3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 57657 Human
    • Entrez Gene: 15168 Mouse
    • Entrez Gene: 114245 Rat
    • Omim: 609973 Human
    • SwissProt: Q9P1Z3 Human
    • SwissProt: O88705 Mouse
    • SwissProt: Q9JKA8 Rat
    • Unigene: 706960 Human
    • Unigene: 389461 Mouse
    • Unigene: 48801 Rat
    see all
  • Alternative names

    • Hcn3 antibody
    • HCN3_HUMAN antibody
    • hyperpolarization activated cyclic nucleotide-gated potassium channel 3 antibody
    • KIAA1535 antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 antibody
    see all

Images

  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 0.511 µg/ml

    Lane 1 : Mouse Brain Tissue
    Lane 2 : Rat Brain Tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 86 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking/Diluting Buffer and concentration: 5% NFDM /TBST 

  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 1/10000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 1/1000 dilution

    Lane 1 : Human forebrain lysate
    Lane 2 : Human hypothalamus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (ab192025)

    Western blot analysis of HCN3 in Human fetal brain lysate immunoprecipitated using ab192025 at 1/30 dilution.

    Secondary antibody:  Goat anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution. 

    Blocking/dilution buffer: 5% NFDM/TBST.

  • Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Anti-HCN3 antibody [EPR13494(2)] (ab192025)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (1)

Publishing research using ab192025? Please let us know so that we can cite the reference in this datasheet.

ab192025 has been referenced in 1 publication.

  • He F  et al. The role of HCN channels in peristaltic dysfunction in human ureteral tuberculosis. Int Urol Nephrol 50:639-645 (2018). WB . PubMed: 29460132

Customer reviews and Q&As

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