Recombinant Anti-HCN3 antibody [EPR13494(2)] (ab192025)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-HCN3 antibody [EPR13494(2)]
See all HCN3 primary antibodies -
Description
Rabbit monoclonal [EPR13494(2)] to HCN3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human HCN3 aa 650 to the C-terminus. The exact sequence is proprietary.
Database link: Q9P1Z3 -
Positive control
- Human cerebellum, fetal brain, forebrain and hypothalamus lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13494(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab192025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa). | |
| IP | 1/30. |
Target
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Function
Putative hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. -
Sequence similarities
Belongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 57657 Human
- Omim: 609973 Human
- SwissProt: Q9P1Z3 Human
- Unigene: 706960 Human
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Alternative names
- Hcn3 antibody
- HCN3_HUMAN antibody
- hyperpolarization activated cyclic nucleotide-gated potassium channel 3 antibody
see all
Images
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![Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)](https://www.abcam.com/ps/products/192/ab192025/Images/ab192025-229390-anti-hcn3-antibody-epr134942-western-blot.jpg)
Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 1/10000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 86 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)](https://www.abcam.com/ps/products/192/ab192025/Images/ab192025-229391-anti-hcn3-antibody-epr134942-western-blot.jpg)
Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 1/1000 dilution
Lane 1 : Human forebrain lysate
Lane 2 : Human hypothalamus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 86 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (ab192025)](https://www.abcam.com/ps/products/192/ab192025/Images/ab192025-229392-anti-hcn3-antibody-epr134942-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (ab192025)Western blot analysis of HCN3 in Human fetal brain lysate immunoprecipitated using ab192025 at 1/30 dilution.
Secondary antibody: Goat anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution.
Blocking/dilution buffer: 5% NFDM/TBST.
Datasheets and documents
References
This product has been referenced in:
- He F et al. The role of HCN channels in peristaltic dysfunction in human ureteral tuberculosis. Int Urol Nephrol 50:639-645 (2018). WB . Read more (PubMed: 29460132) »
