RecombinantRabMAb

Recombinant Anti-HCN3 antibody [EPR13494(2)] (ab192025)

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Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
  • Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
  • Anti-HCN3 antibody [EPR13494(2)] (ab192025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13494(2)] to HCN3
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HCN3 antibody [EPR13494(2)]
    See all HCN3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13494(2)] to HCN3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human HCN3 aa 650 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9P1Z3

  • Positive control

    • WB: Human cerebellum, fetal brain, forebrain and hypothalamus lysates; Mouse and Rat brain tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab192025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
IP 1/30.

Target

Images

  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 0.511 µg/ml

    Lane 1 : Mouse Brain Tissue
    Lane 2 : Rat Brain Tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 86 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting Buffer and concentration: 5% NFDM /TBST 

  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 1/10000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 1/1000 dilution

    Lane 1 : Human forebrain lysate
    Lane 2 : Human hypothalamus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (ab192025)

    Western blot analysis of HCN3 in Human fetal brain lysate immunoprecipitated using ab192025 at 1/30 dilution.

    Secondary antibody:  Goat anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution. 

    Blocking/dilution buffer: 5% NFDM/TBST.

  • Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Anti-HCN3 antibody [EPR13494(2)] (ab192025)

References (1)

Publishing research using ab192025? Please let us know so that we can cite the reference in this datasheet.

ab192025 has been referenced in 1 publication.

  • He F  et al. The role of HCN channels in peristaltic dysfunction in human ureteral tuberculosis. Int Urol Nephrol 50:639-645 (2018). WB . PubMed: 29460132

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