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RecombinantRabMAb

Recombinant Anti-HCN3 antibody [EPR13494(2)] (ab192025)

Submit a review Submit a question References (1)
Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
  • Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
  • Anti-HCN3 antibody [EPR13494(2)] (ab192025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13494(2)] to HCN3
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-HCN3 antibody [EPR13494(2)]
    See all HCN3 primary antibodies

  • Description

    Rabbit monoclonal [EPR13494(2)] to HCN3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human cerebellum, fetal brain, forebrain and hypothalamus lysates; Mouse and Rat brain tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab192025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
IP
1/30.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
IP
1/30.

Target

Images

  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 0.511 µg/ml

    Lane 1 : Mouse Brain Tissue
    Lane 2 : Rat Brain Tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 86 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking/Diluting Buffer and concentration: 5% NFDM /TBST 

  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 1/10000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Western blot - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    All lanes : Anti-HCN3 antibody [EPR13494(2)] (ab192025) at 1/1000 dilution

    Lane 1 : Human forebrain lysate
    Lane 2 : Human hypothalamus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (ab192025)

    Western blot analysis of HCN3 in Human fetal brain lysate immunoprecipitated using ab192025 at 1/30 dilution.

    Secondary antibody:  Goat anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution. 

    Blocking/dilution buffer: 5% NFDM/TBST.

  • Anti-HCN3 antibody [EPR13494(2)] (ab192025)
    Anti-HCN3 antibody [EPR13494(2)] (ab192025)

References (1)

Publishing research using ab192025? Please let us know so that we can cite the reference in this datasheet.

ab192025 has been referenced in 1 publication.

  • He F  et al. The role of HCN channels in peristaltic dysfunction in human ureteral tuberculosis. Int Urol Nephrol 50:639-645 (2018). WB . PubMed: 29460132

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