Overview

  • Product name

    Anti-HCN3 antibody [N141/28]
    See all HCN3 primary antibodies
  • Description

    Mouse monoclonal [N141/28] to HCN3
  • Host species

    Mouse
  • Specificity

    Does not cross react with other HCNs.
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Mouse HCN3 aa 660-779 (C terminal).
    Database link: O88705

  • Positive control

    • Rat brain lysate.
  • General notes

    The clone number has been updated from S141-28 to N141/28, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab84818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 87 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.

Target

Images

  • Anti-HCN3 antibody [N141/28] (ab84818) at 1/1000 dilution + Rat brain membrane lysate

    Predicted band size: 87 kDa

  • ab84818 staining HCN3 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
  • Ab84818 staining HCN3 in Mouse brain tissue sections by Immunohistochemistry. Samples were fixed with 10% Formalin solution at room temperature. Samples were incubated with primary antibody at 1/1000 dilution for 1 hour at room temperature. A HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase was used as the secondary antibody. Mayer Hematoxylin (blue) was used as nuclear counterstain. 

References

This product has been referenced in:

  • Liu Q  et al. Decreased hyperpolarization-activated cyclic nucleotide-gated channels are involved in bladder dysfunction associated with spinal cord injury. Int J Mol Med 41:2609-2618 (2018). Read more (PubMed: 29436607) »
  • Liu Q  et al. Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice. Exp Mol Med 49:e319 (2017). Read more (PubMed: 28428632) »
See all 2 Publications for this product

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