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I would like to speak with some of you about problems with HCN4 antibody that are difficoult to explain by mail. Please can you call me at this number +39 055 4271249 Thank you Best regards
Asked on Jul 27 2006
Thank you for your enquiry. Further to your telephone enquiry I have been in correspondence with the source of this antibody. Unfortunately the immunogen peptide has been exhausted for this antibody and therefore unavailable for purchase. The synthetic peptide used to generate this product corresponds to amino acid residues 1048-1085 of human HCN4 [S(1048) HGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKL (1085)] Bands that are larger than the expected target protein size could be due to a variety of reasons such as: 1. Post translational modification of the protein; phosphorylated, glycosylated, splicing variant, or due to cross-reactivity with the secondary. 2. Reducing agents can also cause target proteins to be visualized at unexpected sizes. From the details that I have an expected molecular weight of 132kDa is expected. This antibody was applied in the following publication; European Journal of Neuroscience, Volume 17:2084-2096, May 2003. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.
Answered on Jul 31 2006