Overview

  • Product name

    Anti-hCNT3 antibody
  • Description

    Rabbit polyclonal to hCNT3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human hCNT3 aa 1-102.
    Database link: Q9HAS3

  • Positive control

    • WB: Mouse lung tissue lysate. IHC: Human colon cancer tissue and testis tissue. ICC/IF: HepG2 cells.
  • General notes

    Protein previously labeled as SLC28A3.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab223085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Sodium-dependent, pyrimidine- and purine-selective. Involved in the homeostasis of endogenous nucleosides. Exhibits the transport characteristics of the nucleoside transport system cib or N3 subtype (N3/cib) (with marked transport of both thymidine and inosine). Employs a 2:1 sodium/nucleoside ratio. Also able to transport gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine.
  • Tissue specificity

    Expressed in pancreas, bone marrow, trachea, mammary gland, liver, prostate, and regions of intestine, brain, lung, placenta, testis, kidney, and heart.
  • Sequence similarities

    Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CNT 3 antibody
    • Concentrative Na(+)-nucleoside cotransporter 3 antibody
    • hCNT3 antibody
    • S28A3_HUMAN antibody
    • Slc28a3 antibody
    • Solute carrier family 28 member 3 antibody
    see all

Images

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for hCNT3 (green) using ab223085 (1/100 dilution) in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin embedded human testis tissue stained for hCNT3 with ab223085 (1/100 dilution) in immunohistochemical analysis.

  • Paraffin embedded human colon cancer tissue stained for hCNT3 with ab223085 (1/100 dilution) in immunohistochemical analysis.

  • Anti-hCNT3 antibody (ab223085) at 1/1000 dilution + mouse lung tissue lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 77 kDa
    Observed band size: 77 kDa

References

ab223085 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223085.
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