Overview

  • Product name

  • Description

    Goat polyclonal to HCP1/PCFT
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human HCP1/PCFT aa 234-247 (internal sequence) (Cysteine residue).
    Sequence:

    LKEPKSTRLFTFRH


    Database link: Q96NT5

  • Positive control

    • Human liver lysate.
  • General notes

    Previously labelled as HCP1.

Properties

Applications

Our Abpromise guarantee covers the use of ab118384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Has been shown to act both as an intestinal proton-coupled high-affinity folate transporter and as an intestinal heme transporter which mediates heme uptake from the gut lumen into duodenal epithelial cells. The iron is then released from heme and may be transported into the bloodstream. Dietary heme iron is an important nutritional source of iron. Shows a higher affinity for folate than heme.
  • Tissue specificity

    Expressed in kidney, liver, placenta, small intestine, spleen, retina and retinal pigment epithelium. Lower levels found in colon and testis. Very low levels in brain, lung, stomach, heart and muscle. In intestine, expressed in duodenum with lower levels in jejunum, ileum, cecum, rectum and segments of the colon.
  • Involvement in disease

    Defects in SLC46A1 are the cause of hereditary folate malabsorption (HFM) [MIM:229050]. HFM is a rare autosomal recessive disorder characterized by impaired intestinal folate absorption with folate deficiency resulting in anemia, hypoimmunoglobulinemia with recurrent infections, and recurrent or chronic diarrhea. In many patients, neurological abnormalities such as seizures or mental retardation become apparent during early childhood, attributed to impaired transport of folates into the central nervous system. When diagnosed early, the disorder can be treated by administration of folate. If untreated, it can be fatal and, if treatment is delayed, the neurological defects can become permanent.
  • Sequence similarities

    Belongs to the major facilitator superfamily. SLC46A family.
  • Cellular localization

    Apical cell membrane. Cytoplasm. Localizes to the apical membrane of intestinal cells in iron-deficient cells, while it resides in the cytoplasm in iron-replete cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • G21 antibody
    • HCP 1 antibody
    • HCP1 antibody
    • Heme carrier protein 1 antibody
    • MGC9564 antibody
    • PCFT antibody
    • PCFT/HCP1 antibody
    • PCFT_HUMAN antibody
    • PDE7A antibody
    • Proton coupled folate transporter antibody
    • Proton-coupled folate transporter antibody
    • SLC46A1 antibody
    • Solute carrier family 46 (folate transporter) member 1 antibody
    • Solute carrier family 46 member 1 antibody
    see all

Images

  • Anti-HCP1/PCFT antibody (ab118384) at 0.3 µg/ml (for 1 hour) + Human Liver lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 50 kDa

References

ab118384 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab25134.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for your reply with further information.

Reviewing the protocol and testing conditions, I do not think that further optimization will improve the results signficantly. You may of received a faulty vial or batch, and I must apologize for any inconvenience this has cuased. We have another HCP1 antibody that is suitable for human samples in WB:

ab98043 Anti-HCP1 antibody
www.abcam.com/ab98043

Would you like to test this product or would you prefer a full refund? Please let me know how you would like to proceed.

Read More

Question
Answer

I am sorry to hear you have been experiencing problems with ab11834.

As we discussed over the phone, please could you send an image of your results and a bit more information about the protocol. Here are some further questions:

- Amount of protein tested

- Has the secondary performance been verified with other primary antibodies

- Washing conditions.

- Sample preparation before loading.

I will review the protocol information and see if there are any suggestions I can make that might improve the results. I would also be happy to replace or refund the product as it is not working as described. I look forward to your reply.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab118384.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

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