Product nameAnti-HCST antibody
DescriptionGoat polyclonal to HCST
SpecificityThis antibody is expected to recognise isoform 1 and may recognize isoform 2 lacking residue E81.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cynomolgus monkey, Macaque monkey
- Human Spleen lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab58596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 9 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHCST is a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. It may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D and may activate phosphatidylinositol 3-kinase dependent signaling pathways. The receptor complex containing HCST may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms.
Cellular localizationCell Membrane
- DAP10 antibody
- DNAX activation protein 10 antibody
- Hematopoietic cell signal transducer antibody
Anti-HCST antibody (ab58596) at 0.2 µg/ml + Human spleen lysate at 35 µg
Predicted band size: 9 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Detected by chemiluminescence.
The additional 100 kDa band of unknown identity was successfully blocked by incubation with the immunising peptide.
ab58596 has not yet been referenced specifically in any publications.