Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)

Abreviews (2)Q&A (7)Specific References (1)

Overview

  • Product name
    Anti-HCV subtype 1b NS5B antibody [10D6]
    See all HCV subtype 1b NS5B primary antibodies
  • Description
    Mouse monoclonal [10D6] to HCV subtype 1b NS5B
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Hepatitis C virus
  • Immunogen

    Recombinant fragment:

    LSSKAVNHIRSVWKDLLEDT

    , corresponding to amino acids 111-130 of HCV subtype 1b NS5B

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Huh7 cells harboring selectable subgenomic HCV RNA replicon

Properties

Applications

Our Abpromise guarantee covers the use of ab100895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 65 kDa.
IP Use at an assay dependent dilution.
ELISA 1/1000.

Target

  • Cellular localization
    Host cytoplasm, nucleus, mitochondrion, endoplasmatic reticulum; secreted.
  • Database links
    • Alternative names
      • Hepacivirin antibody
      • NS3P antibody
      • POLG_HCVBK antibody
      • Serine protease NS3 antibody

    Images

    • Immunoprecipitation - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
      Immunoprecipitation - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
      IP was carried out with ab100895 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (-NS5B) and -Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with ab100895.
    • - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
      - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
      All lanes : Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)

      Lane 1 : Huh-7
      transfected with HCV replicon
      Lane 2 : Huh-7 nontransfected
      cells

      Predicted band size: 65 kDa

    References

    This product has been referenced in:
    • Vogt DA  et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog 9:e1003302 (2013). WB . Read more (PubMed: 23593007) »

    See 1 Publication for this product

    Publishing research using ab100895? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    9 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-HCV subtype 1b NS5B antibody [10D6]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hepatoma cell line)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    30 µg
    Specification
    hepatoma cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted May 16 2016

    Western blot abreview for Anti-HCV subtype 1b NS5B antibody [10D6]

     Good
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Hepatitis C Virus Cell lysate - whole cell (liver carcinoma cell)
    Specification
    liver carcinoma cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted Oct 29 2014


    Thank you for your kind response.

    If so, we are interested in yourHCVNS5B antibody (ab65410) useful for WB and IP. Could you please send it to me right away?

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab65410.

    To check the status of the order please contact o...

    Read More


    Thank you for the information.
    I am looking for a good antibody against HCV NS5B protein for WB and IP experiments. If you have any good antibody for these
    purposes, I want to receive that antibody. Otherwise, I want to be refunded total...

    Read More

    Thanks for your email.

    We do have a few additional HCV NS5B antibodies, but there is not one obvious, best choice. So I will mention 3 that you may find useful.
    ab35586 - most popular anti-HCV NS5B antibody, published in 5 papers. Tested...

    Read More

    hier ist der ausgefüllte Fragebogen.
    Mit freundliche Grüssen,

    1) Abcam product code: ab100895
    2) Abcam order reference number or product batch number:
    3) Description of the problem: Nonspecific binding on Western blot, no spec...

    Read More

    Vielen Dank dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.



    Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten.

    Um Ihr Problem zu lösen, möchte ich die folgenden Vorschläge z...

    Read More

    Sehr geehrtes Abcam Team,
    ich würde Ihr Angebot gerne wahrnehmen und ab100895testen und Ihren 100% Abreview Rabatt in Anspruch nehmen. Natürlich stellen wir Ihnen unsere Ergebnisse mit den Genotyp 2a gerne zur Verfügung.

    DISCOUNT CODE: x
    Ablaufdatum: ddmm2012

    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab100895im Western blot oder der Immunfluoreszenz (IF) an HCV Genotyp 2a zu testen, annehmen möchten. Wie besprochen, ...

    Read More

    Sehr geehrtes Abcam-Team,
    vielen Dank für die Antwort bezüglich des NS5b Antikörpers. Können Sie mir schon sagen, ob eine Kreuzreaktion mit dem HCV Genotyp 2a möglich ist?
    Viele Grüße

    Vielen Dank dafür, dass Sie nachhaken.

    Leider haben wir noch keine guten Nachrichten für Sie:

    Wie versprochen haben wir uns in den entsprechenden Laboren erkundigt. Bislang haben wir für unsere anti-HCV NS5B-Antik&oum...

    Read More

    Dear technical team,   Our customer would like to purchase HCV NS5B antibody which detects 1b or 2a type.   She assume that ab100895 is right for her test but, to make sure check this please.   Thank you. Best regards,

    Thank you for contacting us. I am sorry for the delay in this response. ab100895 is a Mouse monoclonal antibody to Hepatitis C virus NS5B which has been used previously for ELISA and Western blotting. Unfortunately I have been unable to confirm if this...

    Read More

    I have got an additional question concerning the Hepatitis C virus NS5B antibody (ab100895). Can you please tell me the genotype your antibody is based on? i'd like to get NS5B antibody for 1b or 2a type.

    Thank you for your patience on this matter. I have contacted our supplying laboratory on this matter and they have been unable to get back to me with any information regarding this question. I am sorry to say it does not appear that we have an answer f...

    Read More

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