Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)

Reviews (2)Q&A (7)References (2)
Immunoprecipitation - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
  • Western blot - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)

Key features and details

  • Mouse monoclonal [10D6] to HCV subtype 1b NS5B
  • Suitable for: WB, IP, ELISA
  • Reacts with: Hepatitis C virus
  • Isotype: IgG1

Overview

  • Product name

    Anti-HCV subtype 1b NS5B antibody [10D6]
    See all HCV subtype 1b NS5B primary antibodies

  • Description

    Mouse monoclonal [10D6] to HCV subtype 1b NS5B

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IP, ELISAmore details

  • Species reactivity

    Reacts with: Hepatitis C virus

  • Immunogen

    Recombinant fragment:

    LSSKAVNHIRSVWKDLLEDT

    , corresponding to amino acids 111-130 of HCV subtype 1b NS5B
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Huh7 cells harboring selectable subgenomic HCV RNA replicon

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab100895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000. Predicted molecular weight: 65 kDa.
IP
Use at an assay dependent dilution.
ELISA
1/1000.
Notes
WB
1/1000. Predicted molecular weight: 65 kDa.
IP
Use at an assay dependent dilution.
ELISA
1/1000.

Target

  • Cellular localization

    Host cytoplasm, nucleus, mitochondrion, endoplasmatic reticulum; secreted.

  • Database links

    • Alternative names

      • Hepacivirin antibody
      • NS3P antibody
      • POLG_HCVBK antibody
      • Serine protease NS3 antibody

    Images

    • Immunoprecipitation - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
      Immunoprecipitation - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
      IP was carried out with ab100895 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (-NS5B) and -Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with ab100895.
    • Western blot - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
      Western blot - Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
      All lanes : Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)

      Lane 1 : Huh-7
      transfected with HCV replicon
      Lane 2 : Huh-7 nontransfected
      cells

      Predicted band size: 65 kDa

    References (2)

    Publishing research using ab100895? Please let us know so that we can cite the reference in this datasheet.

    ab100895 has been referenced in 2 publications.

    • Chen SR  et al. In Vitro Study on Anti-Hepatitis C Virus Activity of Spatholobus suberectus Dunn. Molecules 21:N/A (2016). PubMed: 27754461
    • Vogt DA  et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog 9:e1003302 (2013). WB . PubMed: 23593007

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-HCV subtype 1b NS5B antibody [10D6]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hepatoma cell line)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    30 µg
    Specification
    hepatoma cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 16 2016

    Western blot abreview for Anti-HCV subtype 1b NS5B antibody [10D6]

     Good
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Hepatitis C Virus Cell lysate - whole cell (liver carcinoma cell)
    Specification
    liver carcinoma cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 29 2014

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