Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
Key features and details
- Mouse monoclonal [10D6] to HCV subtype 1b NS5B
- Suitable for: WB, IP, ELISA
- Reacts with: Hepatitis C virus
- Isotype: IgG1
Overview
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Product name
Anti-HCV subtype 1b NS5B antibody [10D6]
See all HCV subtype 1b NS5B primary antibodies -
Description
Mouse monoclonal [10D6] to HCV subtype 1b NS5B -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, ELISAmore details -
Species reactivity
Reacts with: Hepatitis C virus -
Immunogen
Recombinant fragment:
LSSKAVNHIRSVWKDLLEDT
, corresponding to amino acids 111-130 of HCV subtype 1b NS5B -
Positive control
- Huh7 cells harboring selectable subgenomic HCV RNA replicon
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
10D6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab100895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000. Predicted molecular weight: 65 kDa.
|
IP |
Use at an assay dependent dilution.
|
|
ELISA |
1/1000.
|
Notes |
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WB
1/1000. Predicted molecular weight: 65 kDa. |
IP
Use at an assay dependent dilution. |
ELISA
1/1000. |
Target
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Cellular localization
Host cytoplasm, nucleus, mitochondrion, endoplasmatic reticulum; secreted. -
Database links
- SwissProt: Q9WMX2 Hepatitis C virus
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Alternative names
- Hepacivirin antibody
- NS3P antibody
- POLG_HCVBK antibody
- Serine protease NS3 antibody
Images
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IP was carried out with ab100895 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (-NS5B) and -Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with ab100895.
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All lanes : Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)
Lane 1 : Huh-7
transfected with HCV replicon
Lane 2 : Huh-7 nontransfected
cells
Predicted band size: 65 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab100895 has been referenced in 2 publications.
- Chen SR et al. In Vitro Study on Anti-Hepatitis C Virus Activity of Spatholobus suberectus Dunn. Molecules 21:N/A (2016). PubMed: 27754461
- Vogt DA et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog 9:e1003302 (2013). WB . PubMed: 23593007