Overview

  • Product name
    Anti-HCV subtype 1b NS5B antibody [10D6]
    See all HCV subtype 1b NS5B primary antibodies
  • Description
    Mouse monoclonal [10D6] to HCV subtype 1b NS5B
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Hepatitis C virus
  • Immunogen

    Recombinant fragment:

    LSSKAVNHIRSVWKDLLEDT

    , corresponding to amino acids 111-130 of HCV subtype 1b NS5B

  • Positive control
    • Huh7 cells harboring selectable subgenomic HCV RNA replicon

Properties

Applications

Our Abpromise guarantee covers the use of ab100895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 65 kDa.
IP Use at an assay dependent dilution.
ELISA 1/1000.

Target

  • Cellular localization
    Host cytoplasm, nucleus, mitochondrion, endoplasmatic reticulum; secreted.
  • Database links
    • Alternative names
      • Hepacivirin antibody
      • NS3P antibody
      • POLG_HCVBK antibody
      • Serine protease NS3 antibody

    Images

    • IP was carried out with ab100895 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (-NS5B) and -Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with ab100895.
    • All lanes : Anti-HCV subtype 1b NS5B antibody [10D6] (ab100895)

      Lane 1 : Huh-7
      transfected with HCV replicon
      Lane 2 : Huh-7 nontransfected
      cells

      Predicted band size: 65 kDa

    References

    This product has been referenced in:
    • Vogt DA  et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog 9:e1003302 (2013). WB . Read more (PubMed: 23593007) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hepatoma cell line)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    30 µg
    Specification
    hepatoma cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted May 16 2016

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Hepatitis C Virus Cell lysate - whole cell (liver carcinoma cell)
    Specification
    liver carcinoma cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Oct 29 2014

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