Western blot abreview for Anti-HCV subtype 1b NS5B antibody [10D6]

Good
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Hepatitis C Virus Cell lysate - whole cell (liver carcinoma cell)
Specification
liver carcinoma cell
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
1 hour(s) and 30 minute(s) · Diluent: TBST
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-mouse IgG Peroxidase antibody produced in goa
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 66 kDa
Exposure
1 minute(s) and 0 second(s)
Positive control
-

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Submitted Oct 29 2014

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