Western blot abreview for Anti-HCV subtype 1b NS5B antibody [10D6]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (hepatoma cell line)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
30 µg
Specification
hepatoma cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
1 hour(s) and 30 minute(s) · Diluent: TBST
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: anti-mouse IgG peroxidase antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 66 kDa

Abcam user community

Verified customer

Submitted May 16 2016

Sign up