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  1. Link

    hcv-subtype-1b-ns5b-antibody-ab203074.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HCV
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Anti-HCV subtype 1b NS5B antibody (ab203074)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCV subtype 1b NS5B antibody (ab203074)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCV subtype 1b NS5B antibody (ab203074)

Key features and details

  • Rabbit polyclonal to HCV subtype 1b NS5B
  • Suitable for: IHC-P
  • Reacts with: Hepatitis C virus
  • Isotype: IgG

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Overview

  • Product name

    Anti-HCV subtype 1b NS5B antibody
    See all HCV subtype 1b NS5B primary antibodies
  • Description

    Rabbit polyclonal to HCV subtype 1b NS5B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Hepatitis C virus
  • Immunogen

    Synthetic peptide within Hepatitis C virus HCV subtype 1b NS5B aa 1400-1550 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Hepatitis C virus genotype 1b (isolate Con1).
    Database link: Q9WMX2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Mouse liver and rat colitis tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HCV

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab203074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Cellular localization

    Host cytoplasm, nucleus, mitochondrion, endoplasmatic reticulum; secreted.
  • Database links

    • SwissProt: Q9WMX2 Hepatitis C virus
    • Alternative names

      • Hepacivirin antibody
      • NS3P antibody
      • POLG_HCVBK antibody
      • Serine protease NS3 antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCV subtype 1b NS5B antibody (ab203074)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCV subtype 1b NS5B antibody (ab203074)

      Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse liver tissue, labeling HCV subtype 1b NS5B using ab203074 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCV subtype 1b NS5B antibody (ab203074)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCV subtype 1b NS5B antibody (ab203074)

      Immunohistochemical analysis of formalin-fixed paraffin-embedded rat colitis tissue, labeling HCV subtype 1b NS5B using ab203074 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab203074? Please let us know so that we can cite the reference in this datasheet.

    ab203074 has not yet been referenced specifically in any publications.

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