Overview

  • Product name
    Anti-HCV subtype 1b NS5B antibody
    See all HCV subtype 1b NS5B primary antibodies
  • Description
    Rabbit polyclonal to HCV subtype 1b NS5B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Hepatitis C virus
  • Immunogen

    Recombinant fragment (Hepatitis C Virus) with a 21 amino acid C-terminal truncation

Properties

Applications

Our Abpromise guarantee covers the use of ab35586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/2000. Detects a band of approximately 64 kDa.

Target

  • Cellular localization
    Host cytoplasm, nucleus, mitochondrion, endoplasmatic reticulum; secreted.
  • Database links
    • Alternative names
      • Hepacivirin antibody
      • NS3P antibody
      • POLG_HCVBK antibody
      • Serine protease NS3 antibody

    Images

    • All lanes : Anti-HCV subtype 1b NS5B antibody (ab35586) at 1/2000 dilution

      Lane 1 : Lysate from Huh7 cells, mock transfection
      Lane 2 : Lysate from Huh7 cells transfected with NS5B

      Observed band size: 64 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:
    • Zhao Q  et al. Dual Roles of Two Isoforms of Autophagy-related Gene ATG10 in HCV-Subgenomic replicon Mediated Autophagy Flux and Innate Immunity. Sci Rep 7:11250 (2017). Read more (PubMed: 28900156) »
    • Vegna S  et al. NOD1 Participates in the Innate Immune Response Triggered by Hepatitis C Virus Polymerase. J Virol 90:6022-35 (2016). Read more (PubMed: 27099311) »
    See all 14 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Application
    ELISA
    Sample
    Human Cell (huh-7 hepatocarcinoma cells)
    Specification
    huh-7 hepatocarcinoma cells
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
    Type
    Sandwich (Capture)

    Abcam user community

    Verified customer

    Submitted Jun 17 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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