Key features and details
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Inhibitor compounds
Product nameHDAC Inhibitor Drug Screening Kit (Fluorometric)
See all HDAC kits
Sample typeInhibitor compounds
Assay durationMultiple steps standard assay
Inhibition of histone deacetylase (HDAC) has been implicated to modulate transcription, to induce apoptosis or differentiation in cancer cells. However, screening of compounds for HDAC inhibition has been difficult due to the lack of convenient tools for analyzing HDAC activity. The new HDAC Inhibitor Drug Screening Kit provides a fast, fluorescence-based method that eliminates radioactivity, extractions, or chromatography, as used in traditional assays. The new procedure requires only two easy steps, both performed on the same microtiter plate. First, your inhibitor candidates are mixed with HeLa Nuclear Extract and HDAC fluorometric substrate, which comprises an acetylated lysine side chain. Deacetylation of the substrate sensitizes the substrate, so that, in the second step, treatment with the Lysine Developer produces a fluorophore. The fluorophore can be easily analyzed using a fluorescence plate reader or a fluorometer. The assay is well suited for high throughput screening applications.
This product is manufactured by BioVision, an Abcam company and was previously called K340 HDAC Inhibitor Drug Screening Kit (Fluorometric). K340-100 is the same size as the 100 test size of ab283378.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests 10X HDAC Assay Buffer Green 1 x 1ml HDAC Inhibitor Blue 1 x 10µl HDAC Substrate (Boc-Lys(Ac)-AMC, 4 mM) Amber 1 x 500µl HeLa Nuclear Extract (5 mg/ml) Red 1 x 200µl Lysine Developer Orange 1 x 1ml
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Component a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes. Phosphorylated by CDK5.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by KCTD11, leading to proteasomal degradation.
- Information by UniProt
- Histone deacetylase 1
ab283378 has not yet been referenced specifically in any publications.